Membrane-assisted viral DNA ejection

Show simple item record Santos-Perez, Isaac Oksanen, Hanna M. Bamford, Dennis H. Goni, Felix M. Reguera, David Abrescia, Nicola G. A. 2018-03-01T22:12:01Z 2021-12-17T18:47:45Z 2017-03
dc.identifier.citation Santos-Perez , I , Oksanen , H M , Bamford , D H , Goni , F M , Reguera , D & Abrescia , N G A 2017 , ' Membrane-assisted viral DNA ejection ' , Biochimica et Biophysica Acta. General Subjects , vol. 1861 , no. 3 , pp. 664-672 .
dc.identifier.other PURE: 83096836
dc.identifier.other PURE UUID: daf32711-1b14-42be-a618-f5d8e289a069
dc.identifier.other WOS: 000394076400018
dc.identifier.other Scopus: 85009266789
dc.identifier.other ORCID: /0000-0002-6438-8118/work/50861385
dc.identifier.other ORCID: /0000-0003-3047-8294/work/50886769
dc.description.abstract Genome packaging and delivery are fundamental steps in the replication cycle of all viruses. Icosahedral viruses with linear double-stranded DNA (dsDNA) usually pacicage their genome into a preformed, rigid procapsid using the power generated by a virus-encoded packaging ATPase. The pressure and stored energy due to this confinement of DNA at a high density is assumed to drive the initial stages of genome ejection. Membrane-containing icosahedral viruses, such as bacteriophage PRD1, present an additional architectural complexity by enclosing their genome within an internal membrane vesicle. Upon adsorption to a host cell, the PRD1 membrane remodels into a proteo-lipidic tube that provides a conduit for passage of the ejected linear dsDNA through the cell envelope. Based on volume analyses of PRD1 membrane vesicles captured by cryo-electron tomography and modeling of the elastic properties of the vesicle, we propose that the internal membrane makes a crucial and active contribution during infection by maintaining the driving force for DNA ejection and countering the internal turgor pressure of the host These novel functions extend the role of the PRD1 viral membrane beyond tube formation or the mere physical confinement of the genome. The presence and assistance of an internal membrane might constitute a biological advantage that extends also to other viruses that package their linear dsDNA to high density within an internal vesicle. (C) 2016 Elsevier B.V. All rights reserved. en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof Biochimica et Biophysica Acta. General Subjects
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Models of viral DNA packaging
dc.subject Viral DNA delivery
dc.subject Lipid-containing viruses
dc.subject Membrane vesicle and properties
dc.subject Biophysical modeling
dc.subject Cryo-electron tomography
dc.subject FORCES
dc.subject PHAGE
dc.subject VIRUSES
dc.subject CAPSIDS
dc.subject IMAGE
dc.subject 1183 Plant biology, microbiology, virology
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Membrane-assisted viral DNA ejection en
dc.type Article
dc.contributor.organization Biosciences
dc.contributor.organization Institute of Biotechnology
dc.contributor.organization Molecular Principles of Viruses
dc.contributor.organization Structure of the Viral Universe
dc.description.reviewstatus Peer reviewed
dc.relation.issn 0304-4165
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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