MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro

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http://hdl.handle.net/10138/233264

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Korvala , J , Jee , K , Porkola , E , Almangush , A , Mosakhani , N , Bitu , C , Cervigne , N K , Zandonadi , F S , Meirelles , G V , Paes Leme , A F , Coletta , R D , Leivo , I & Salo , T 2017 , ' MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro ' , Experimental Cell Research , vol. 350 , no. 1 , pp. 9-18 . https://doi.org/10.1016/j.yexcr.2016.10.015

Title: MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro
Author: Korvala, Johanna; Jee, Kowan; Porkola, Emmi; Almangush, Alhadi; Mosakhani, Neda; Bitu, Carolina; Cervigne, Nilva K.; Zandonadi, Flavia S.; Meirelles, Gabriela V.; Paes Leme, Adriana Franco; Coletta, Ricardo D.; Leivo, Ilmo; Salo, Tuula
Other contributor: University of Helsinki, Department of Pathology
University of Helsinki, Department of Pathology
University of Helsinki, Department of Pathology
University of Helsinki, Medicum
University of Helsinki, Clinicum





Date: 2017-01-01
Language: eng
Number of pages: 10
Belongs to series: Experimental Cell Research
ISSN: 0014-4827
DOI: https://doi.org/10.1016/j.yexcr.2016.10.015
URI: http://hdl.handle.net/10138/233264
Abstract: Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated "Focal adhesion" and "ECM-receptor interaction" as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren't significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.
Subject: MicroRNA
Proteomics
Oral tongue squamous cell carcinoma
Bioinformatics
OVARIAN-CANCER CELLS
BREAST-CANCER
HEPATOCELLULAR-CARCINOMA
GENE-EXPRESSION
POOR-PROGNOSIS
NECK-CANCER
TUMOR
MIGRATION
GROWTH
METASTASIS
3122 Cancers
313 Dentistry
3111 Biomedicine
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