To involvement the conformation of the adenine nucleotide translocase in opening the Tl+-induced permeability transition pore in Ca2+-loaded rat liver mitochondria

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http://hdl.handle.net/10138/233305

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Korotkov , S M , Konovalova , S A , Brailovskaya , I V & Saris , N-E L 2016 , ' To involvement the conformation of the adenine nucleotide translocase in opening the Tl+-induced permeability transition pore in Ca2+-loaded rat liver mitochondria ' Toxicology in Vitro , vol 32 , pp. 320-332 . DOI: 10.1016/j.tiv.2016.01.015

Julkaisun nimi: To involvement the conformation of the adenine nucleotide translocase in opening the Tl+-induced permeability transition pore in Ca2+-loaded rat liver mitochondria
Tekijä: Korotkov, Sergey M.; Konovalova, Svetlana A.; Brailovskaya, Irina V.; Saris, Nils-Erik L.
Muu tekijä: University of Helsinki, Department of Food and Nutrition
Kuuluu julkaisusarjaan: Toxicology in Vitro
ISSN: 0887-2333
Tiivistelmä: The conformation of adenine nucleotide translocase (ANT) has a profound impact in opening the mitochondrial permeability transition pore (MPTP) in the inner membrane. Fixing the ANT in 'c' conformation by phenylarsine oxide (PAO), tert-butylhydroperoxide (tBHP), and carboxyatractyloside as well as the interaction of 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) with mitochondrial thiols markedly attenuated the ability of ADP to inhibit the MPTP opening. We earlier found (Korotkov and Saris, 2011) that calcium load of rat liver mitochondria in medium containing TINO3 and KNO3 stimulated the Tl+-induced MPTP opening in the inner mitochondrial membrane. The MPTP opening as well as followed increase in swelling, a drop in membrane potential (Delta Psi(mito)), and a decrease in state 3, state 4, and 2,4-dinitrophenol-uncoupled respiration were visibly enhanced in the presence of PAO, tBHP, DIDS, and carboxyatractyloside. However, these effects were markedly inhibited by ADP and membrane-penetrant hydrophobic thiol reagent, N-ethylmaleimide (NEM) which fix the ANT in 'm' conformation. Cyclosporine A additionally potentiated these effects of ADP and NEM. Our data suggest that conformational changes of the ANT may be directly involved in the opening of the Tl+-induced MPTP in the inner membrane of Ca2+-loaded rat liver mitochondria. Using the Tl+-induced MPTP model is discussed in terms finding new transition pore inhibitors and inducers among different chemical and natural compounds. (C) 2016 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/10138/233305
Päiväys: 2016-04
Avainsanat: Tl+
Ca2+
Mitochondrial permeability transition
Adenine nucleotide translocase conformation
Mitochondrial swelling
Rat liver mitochondria
HEART-MITOCHONDRIA
POTASSIUM CHANNELS
THALLIUM TOXICITY
SULFHYDRYL-GROUPS
OXIDATIVE STRESS
ION-TRANSPORT
CYCLOPHILIN-D
MEMBRANE PERMEABILIZATION
LIPID-PEROXIDATION
N-ETHYLMALEIMIDE
1182 Biochemistry, cell and molecular biology
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