Effect of Silene vulgaris and Heavy Metal Pollution on Soil; Microbial Diversity in Long-Term Contaminated Soil

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Pacwa-Plociniczak , M , Plociniczak , T , Yu , D , Kurola , J M , Sinkkonen , A T , Piotrowska-Seget , Z & Romantschuk , M L 2018 , ' Effect of Silene vulgaris and Heavy Metal Pollution on Soil; Microbial Diversity in Long-Term Contaminated Soil ' , Water, Air and Soil Pollution , vol. 229 , no. 1 , 13 . https://doi.org/10.1007/s11270-017-3655-3

Title: Effect of Silene vulgaris and Heavy Metal Pollution on Soil; Microbial Diversity in Long-Term Contaminated Soil
Author: Pacwa-Plociniczak, Magdalena; Plociniczak, Tomasz; Yu, Dan; Kurola, Jukka Mikael; Sinkkonen, Aki Tapio; Piotrowska-Seget, Zofia; Romantschuk, Martin L.
Contributor: University of Helsinki, Environmental Sciences
University of Helsinki, Environmental Sciences
University of Helsinki, Nature-Based Solutions
University of Helsinki, Martin Romantschuk / Principal Investigator
Date: 2018-01
Language: eng
Number of pages: 13
Belongs to series: Water, Air and Soil Pollution
ISSN: 0049-6979
URI: http://hdl.handle.net/10138/233650
Abstract: In this study, we analysed the impact of heavy metals and plant rhizodeposition on the structure of indigenous microbial communities in rhizosphere and bulk soil that had been exposed to heavy metals for more than 150 years. Samples of the rhizosphere of Silene vulgaris and non-rhizosphere soils 250 and 450 m from the source of emission that had different metal concentrations were collected for analyses. The results showed that soils were collected 250 m from the smelter had a higher number of Cd-resistant CFU compared with the samples that were collected from 450 m, but no significant differences were observed in the number of total and oligotrophic CFU or the equivalent cell numbers between rhizosphere and non-rhizosphere soils that were taken 250 and 450 m from the emitter. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis of the denaturing gradient gel electrophoresis (DGGE) profiles, as well as a cluster analysis that was generated on the phospholipid fatty acid (PLFA) profiles, showed that the bacterial community structure of rhizosphere soils depended more on the plant than on the distance and metal concentrations. The sequencing of the 16S rDNA fragments that were excised from the DGGE gel revealed representatives of the phyla Bacteroidetes, Acidobacteria, Gemmatimonadetes, Actinobacteria and Betaproteobacteria in the analysed soil with a predominance of the first three groups. The obtained results demonstrated that the presence of S. vulgaris did not affect the number of CFUs, except for those of Cd-resistant bacteria. However, the presence of S. vulgaris altered the soil bacterial community structure, regardless of the sampling site, which supported the thesis that plants have a higher impact on soil microbial community than metal contamination.
Subject: 1183 Plant biology, microbiology, virology
Heavy metals . Soil pollution . Silene
Heavy metals
Soil pollution
Silene vulgaris
Bacterial diversity
Real-time PCR
GRADIENT GEL-ELECTROPHORESIS
16S RIBOSOMAL-RNA
FATTY-ACID-COMPOSITION
BACTERIAL COMMUNITY
RHIZOSPHERE SOIL
ARABLE SOIL
GENES
TOLERANCE
SEQUENCE
BIOMASS
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