Cancer stem cells from human glioblastoma resemble but do not mimic original tumors after in vitro passaging in serum-free media

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Garcia-Romero , N , Gonzalez-Tejedo , C , Carrion-Navarro , J , Esteban-Rubio , S , Rackov , G , Rodriguez-Fanjul , V , Oliver-De La Cruz , J , Prat-Acin , R , Peris-Celda , M , Blesa , D , Ramirez-Jimenez , L , Sanchez-Gomez , P , Perona , R , Escobedo-Lucea , C , Belda-Iniesta , C & Ayuso-Sacido , A 2016 , ' Cancer stem cells from human glioblastoma resemble but do not mimic original tumors after in vitro passaging in serum-free media ' , Oncotarget , vol. 7 , no. 40 , pp. 65888-65901 . https://doi.org/10.18632/oncotarget.11676

Title: Cancer stem cells from human glioblastoma resemble but do not mimic original tumors after in vitro passaging in serum-free media
Author: Garcia-Romero, Noemi; Gonzalez-Tejedo, Carmen; Carrion-Navarro, Josefa; Esteban-Rubio, Susana; Rackov, Gorjana; Rodriguez-Fanjul, Vanessa; Oliver-De La Cruz, Jorge; Prat-Acin, Ricardo; Peris-Celda, Maria; Blesa, David; Ramirez-Jimenez, Laura; Sanchez-Gomez, Pilar; Perona, Rosario; Escobedo-Lucea, Carmen; Belda-Iniesta, Cristobal; Ayuso-Sacido, Angel
Other contributor: University of Helsinki, Division of Pharmaceutical Biosciences

Date: 2016-10-04
Language: eng
Number of pages: 14
Belongs to series: Oncotarget
ISSN: 1949-2553
DOI: https://doi.org/10.18632/oncotarget.11676
URI: http://hdl.handle.net/10138/233904
Abstract: Human gliomas harbour cancer stem cells (CSCs) that evolve along the course of the disease, forming highly heterogeneous subpopulations within the tumour mass. These cells possess self-renewal properties and appear to contribute to tumour initiation, metastasis and resistance to therapy. CSC cultures isolated from surgical samples are considered the best preclinical in vitro model for primary human gliomas. However, it is not yet well characterized to which extent their biological and functional properties change during in vitro passaging in the serum-free culture conditions. Here, we demonstrate that our CSC-enriched cultures harboured from one to several CSC clones from the human glioma sample. When xenotransplanted into mouse brain, these cells generated tumours that reproduced at least three different dissemination patterns found in original tumours. Along the passages in culture, CSCs displayed increased expression of stem cell markers, different ratios of chromosomal instability events, and a varied response to drug treatment. Our findings highlight the need for better characterization of CSC-enriched cultures in the context of their evolution in vitro, in order to uncover their full potential as preclinical models in the studies aimed at identifying molecular biomarkers and developing new therapeutic approaches of human gliomas.
Subject: cancer stem cells
glioblastoma
genetic alterations
drug discovery
primary cell culture
CENTRAL-NERVOUS-SYSTEM
GLIOMA
IDENTIFICATION
HETEROGENEITY
EPIDEMIOLOGY
TEMOZOLOMIDE
PRECURSORS
PHENOTYPE
PROFILES
GENOTYPE
317 Pharmacy
3122 Cancers
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