Identification of matrix metalloproteinase-2 and-9 activities within the intestinal mucosa of dogs with chronic enteropathies

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Hanifeh , M , Rajamäki , M M , Syrjä , P , Mäkitalo , L , Kilpinen , S & Spillmann , T 2018 , ' Identification of matrix metalloproteinase-2 and-9 activities within the intestinal mucosa of dogs with chronic enteropathies ' , Acta Veterinaria Scandinavica , vol. 60 , 16 . https://doi.org/10.1186/s13028-018-0371-y

Title: Identification of matrix metalloproteinase-2 and-9 activities within the intestinal mucosa of dogs with chronic enteropathies
Author: Hanifeh, Mohsen; Rajamäki, Minna Marjaana; Syrjä, Pernilla; Mäkitalo, Laura; Kilpinen, Susanne; Spillmann, Thomas
Contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Minna Marjaana Rajamäki / Principal Investigator
University of Helsinki, Veterinary Pathology and Parasitology
University of Helsinki, Equine and Small Animal Medicine
University of Helsinki, Thomas Spillmann / Principal Investigator
Date: 2018-03-12
Language: eng
Number of pages: 10
Belongs to series: Acta Veterinaria Scandinavica
ISSN: 0044-605X
URI: http://hdl.handle.net/10138/233939
Abstract: Background: Matrix metalloproteinases (MMPs) 2 and 9 are zinc-and calcium-dependent endopeptidases involved in the breakdown and reconstitution of extracellular matrix under both physiological and pathological conditions. Mucosal MMP-2 and -9 activities have been reported to be upregulated in the intestine of humans with inflammatory bowel disease (IBD), and in animal models of IBD. However, their involvement in the pathogenesis of canine chronic enteropathies (CE) is unknown. This study investigated mucosal pro-and active MMP-2 and -9 activities in dogs with CE and healthy dogs using gelatin zymography, and also to determine the association of their activities in dogs with CE with the canine IBD activity index (CIBDAI), histopathologic findings, the clinical outcome, and hypoalbuminemia. Intestinal mucosal samples from duodenum, ileum, colon, and cecum were collected from 40 dogs with CE and 18 healthy Beagle dogs. Results: In dogs with CE, the number of samples positive for mucosal pro-and active MMP-2 was significantly higher in the duodenum (P <0.0001 and P = 0.011, respectively), ileum (P = 0.002 and P = 0.018, respectively), and colon (P <0.0001 and P = 0.002, respectively), compared with healthy controls. Mucosal pro-MMP-9-positive samples in the duodenum and colon were significantly more frequent in dogs with CE than in healthy dogs (P = 0.0004 and P = 0.001, respectively). Despite the presence of mucosal samples positive for active MMP-9 in the intestinal segments of dogs with CE, the difference compared to healthy controls did not reach statistical significance. None of the intestinal mucosal samples in healthy dogs showed gelatinolytic activity corresponding to the control bands of active MMP-2 and -9. Mucosal active MMP-9 activities displayed a significant positive association with the severity of neutrophil infiltration in the duodenum (P = 00.040), eosinophils in the cecum (P = 00.037), and the CIBDAI score for ileum samples (P = 0.023). There was no significant association of pro-and active MMP-2 and -9 levels with the clinical outcome or hypoalbuminemia. Conclusions: This study is the first to demonstrate upregulation of mucosal pro-and active MMP-2 and pro-MMP-9 in the intestine of dogs with CE compared to healthy dogs. The results provide supporting evidence for the possible involvement of MMP-2 and -9 in the pathogenesis of canine CE.
Subject: Chronic enteropathies
Dog
Matrix metalloproteinase 2
Matrix metalloproteinase 9
Zymography
INFLAMMATORY-BOWEL-DISEASE
GASTROINTESTINAL INFLAMMATION
CROHNS-DISEASE
EXPERIMENTAL COLITIS
ULCERATIVE-COLITIS
GENE-EXPRESSION
BIOPSY SAMPLES
INHIBITORS
TISSUE
DIAGNOSIS
413 Veterinary science
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