Clinical use of fungal PCR from deep tissue samples in the diagnosis of invasive fungal diseases : a retrospective observational study

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http://hdl.handle.net/10138/233989

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Ala-Houhala , M , Koukila-Kahkola , P , Antikainen , J , Valve , J , Kirveskari , J & Anttila , V -J 2018 , ' Clinical use of fungal PCR from deep tissue samples in the diagnosis of invasive fungal diseases : a retrospective observational study ' , Clinical Microbiology and Infection , vol. 24 , no. 3 , pp. 301-305 . https://doi.org/10.1016/j.cmi.2017.08.017

Title: Clinical use of fungal PCR from deep tissue samples in the diagnosis of invasive fungal diseases : a retrospective observational study
Author: Ala-Houhala, M.; Koukila-Kahkola, P.; Antikainen, Jenni; Valve, J.; Kirveskari, J.; Anttila, V. -J.
Contributor: University of Helsinki, HUSLAB
University of Helsinki, HUSLAB
University of Helsinki, Department of Diagnostics and Therapeutics
University of Helsinki, Infektiosairauksien yksikkö
Date: 2018-03
Language: eng
Number of pages: 5
Belongs to series: Clinical Microbiology and Infection
ISSN: 1198-743X
URI: http://hdl.handle.net/10138/233989
Abstract: Objectives: To assess the clinical use of panfungal PCR for diagnosis of invasive fungal diseases (IFDs). We focused on the deep tissue samples. Methods: We first described the design of panfungal PCR, which is in clinical use at Helsinki University Hospital. Next we retrospectively evaluated the results of 307 fungal PCR tests performed from 2013 to 2015. Samples were taken from normally sterile tissues and fluids. The patient population was nonselected. We classified the likelihood of IFD according to the criteria of the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG), comparing the fungal PCR results to the likelihood of IFD along with culture and microscopy results. Results: There were 48 positive (16%) and 259 negative (84%) PCR results. The sensitivity and specificity of PCR for diagnosing IFDs were 60.5% and 91.7%, respectively, while the negative predictive value and positive predictive value were 93.4% and 54.2%, respectively. The concordance between the PCR and the culture results was 86% and 87% between PCR and microscopy, respectively. Of the 48 patients with positive PCR results, 23 had a proven or probable IFD. Conclusions: Fungal PCR can be useful for diagnosing IFDs in deep tissue samples. It is beneficial to combine fungal PCR with culture and microscopy. M. Ala-Houhala, Clin Microbiol Infect 2018;24:301 (C) 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
Subject: Deep tissue samples
diagnosis
fungal PCR
invasive fungal disease
POLYMERASE-CHAIN-REACTION
CELL TRANSPLANT RECIPIENTS
INFECTIONS
EPIDEMIOLOGY
CANDIDEMIA
ASPERGILLOSIS
METAANALYSIS
UTILITY
COSTS
ASSAY
3121 General medicine, internal medicine and other clinical medicine
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