Diversity of luciferase sequences and bioluminescence production in Baltic Sea Alexandrium ostenfeldii

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Le Tortorec , A H , Tahvanainen , P , Kremp , A & Simis , S G H 2016 , ' Diversity of luciferase sequences and bioluminescence production in Baltic Sea Alexandrium ostenfeldii ' European Journal of Phycology , vol. 51 , no. 3 , pp. 317-327 . DOI: 10.1080/09670262.2016.1160441

Title: Diversity of luciferase sequences and bioluminescence production in Baltic Sea Alexandrium ostenfeldii
Author: Le Tortorec, Anniina H.; Tahvanainen, Pia; Kremp, Anke; Simis, Stefan G. H.
Contributor: University of Helsinki, Environmental Sciences
University of Helsinki, Kasvimuseo (-2009)
Date: 2016
Language: eng
Number of pages: 11
Belongs to series: European Journal of Phycology
ISSN: 0967-0262
URI: http://hdl.handle.net/10138/234082
Abstract: The toxic dinoflagellate Alexandrium ostenfeldii is the only bioluminescent bloom-forming phytoplankton in coastal waters of the Baltic Sea. We analysed partial luciferase gene (lcf) sequences and bioluminescence production in Baltic A. ostenfeldii bloom populations to assess the distribution and consistency of the trait in the Baltic Sea, and to evaluate applications for early detection of toxic blooms. Lcf was consistently present in 61 Baltic Sea A. ostenfeldii strains isolated from six separate bloom sites. All Baltic Sea strains except one produced bioluminescence. In contrast, the presence of lcf and the ability to produce bioluminescence did vary among strains from other parts of Europe. In phylogenetic analyses, lcf sequences of Baltic Sea strains clustered separately from North Sea strains, but variation between Baltic Sea strains was not sufficient to distinguish between bloom populations. Clustering of the lcf marker was similar to internal transcribed spacer (ITS) sequences with differences being minor and limited to the lowest hierarchical clusters, indicating a similar rate of evolution of the two genes. In relation to monitoring, the consistent presence of lcf and close coupling of lcf with bioluminescence suggests that bioluminescence can be used to reliably monitor toxic bloom-forming A. ostenfeldii in the Baltic Sea.
Subject: Alexandrium ostenfeldii
bioluminescence
dinoflagellate
harmful algal bloom
internal transcribed spacer
luciferase gene
phylogeny
paralytic shellfish poisoning toxins
DINOFLAGELLATE LINGULODINIUM-POLYEDRUM
PARALYTIC SHELLFISH TOXINS
IRISH COASTAL WATERS
MOLECULAR EVOLUTION
GONYAULAX-POLYEDRA
PHYLOGENETIC-RELATIONSHIPS
NOCTILUCA-SCINTILLANS
EXPRESSED PROTEINS
SINGLE POLYPEPTIDE
PERUVIANUM BALECH
1184 Genetics, developmental biology, physiology
1172 Environmental sciences
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