Allosteric effects of chromophore interaction with dimeric near-infrared fluorescent proteins engineered from bacterial phytochromes

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Stepanenko , O V , Baloban , M , Bublikov , G S , Shcherbakova , D M , Stepanenko , O V , Turoverov , K K , Kuznetsova , I M & Verkhusha , V V 2016 , ' Allosteric effects of chromophore interaction with dimeric near-infrared fluorescent proteins engineered from bacterial phytochromes ' , Scientific Reports , vol. 6 , 18750 . https://doi.org/10.1038/srep18750

Title: Allosteric effects of chromophore interaction with dimeric near-infrared fluorescent proteins engineered from bacterial phytochromes
Author: Stepanenko, Olesya V.; Baloban, Mikhail; Bublikov, Grigory S.; Shcherbakova, Daria M.; Stepanenko, Olga V.; Turoverov, Konstantin K.; Kuznetsova, Irina M.; Verkhusha, Vladislav Vitaliyevich
Other contributor: University of Helsinki, Medicum
Date: 2016-01-04
Language: eng
Number of pages: 13
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/srep18750
URI: http://hdl.handle.net/10138/234121
Abstract: Fluorescent proteins (FPs) engineered from bacterial phytochromes attract attention as probes for in vivo imaging due to their near-infrared (NIR) spectra and use of available in mammalian cells biliverdin (BV) as chromophore. We studied spectral properties of the iRFP670, iRFP682 and iRFP713 proteins and their mutants having Cys residues able to bind BV either in both PAS (Cys15) and GAF (Cys256) domains, in one of these domains, or without these Cys residues. We show that the absorption and fluorescence spectra and the chromophore binding depend on the location of the Cys residues. Compared with NIR FPs in which BV covalently binds to Cys15 or those that incorporate BV noncovalently, the proteins with BV covalently bound to Cys256 have blue-shifted spectra and higher quantum yield. In dimeric NIR FPs without Cys15, the covalent binding of BV to Cys256 in one monomer allosterically inhibits the covalent binding of BV to the other monomer, whereas the presence of Cys15 allosterically promotes BV binding to Cys256 in both monomers. The NIR FPs with both Cys residues have the narrowest blue-shifted spectra and the highest quantum yield. Our analysis resulted in the iRFP713/Val256Cys protein with the highest brightness in mammalian cells among available NIR FPs.
Subject: BILIVERDIN CHROMOPHORE
LIGHT
BACTERIOPHYTOCHROMES
BIOSYNTHESIS
MECHANISM
DOMAIN
AGP1
1182 Biochemistry, cell and molecular biology
319 Forensic science and other medical sciences
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