A Genome-Wide Association Study for Host Resistance to Ostreid Herpesvirus in Pacific Oysters (Crassostrea gigas)

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http://hdl.handle.net/10138/234430

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Gutierrez , A P , Bean , T P , Hooper , C , Stenton , C A , Sanders , M B , Paley , R K , Rastas , P , Bryrom , M , Matika , O & Houston , R D 2018 , ' A Genome-Wide Association Study for Host Resistance to Ostreid Herpesvirus in Pacific Oysters (Crassostrea gigas) ' , G3 - Genes genomes genetics , vol. 8 , no. 4 , pp. 1273-1280 . https://doi.org/10.1534/g3.118.200113

Julkaisun nimi: A Genome-Wide Association Study for Host Resistance to Ostreid Herpesvirus in Pacific Oysters (Crassostrea gigas)
Tekijä: Gutierrez, Alejandro P.; Bean, Tim P.; Hooper, Chantelle; Stenton, Craig A.; Sanders, Matthew B.; Paley, Richard K.; Rastas, Pasi; Bryrom, Michaela; Matika, Oswald; Houston, Ross D.
Tekijän organisaatio: Biosciences
Päiväys: 2018-04
Kieli: eng
Sivumäärä: 8
Kuuluu julkaisusarjaan: G3 - Genes genomes genetics
ISSN: 2160-1836
DOI-tunniste: https://doi.org/10.1534/g3.118.200113
URI: http://hdl.handle.net/10138/234430
Tiivistelmä: Ostreid herpesvirus (OsHV) can cause mass mortality events in Pacific oyster aquaculture. While various factors impact on the severity of outbreaks, it is clear that genetic resistance of the host is an important determinant of mortality levels. This raises the possibility of selective breeding strategies to improve the genetic resistance of farmed oyster stocks, thereby contributing to disease control. Traditional selective breeding can be augmented by use of genetic markers, either via marker-assisted or genomic selection. The aim of the current study was to investigate the genetic architecture of resistance to OsHV in Pacific oyster, to identify genomic regions containing putative resistance genes, and to inform the use of genomics to enhance efforts to breed for resistance. To achieve this, a population of approximate to 1,000 juvenile oysters were experimentally challenged with a virulent form of OsHV, with samples taken from mortalities and survivors for genotyping and qPCR measurement of viral load. The samples were genotyped using a recently-developed SNP array, and the genotype data were used to reconstruct the pedigree. Using these pedigree and genotype data, the first high density linkage map was constructed for Pacific oyster, containing 20,353 SNPs mapped to the ten pairs of chromosomes. Genetic parameters for resistance to OsHV were estimated, indicating a significant but low heritability for the binary trait of survival and also for viral load measures (h2 0.12 - 0.25). A genome-wide association study highlighted a region of linkage group 6 containing a significant QTL affecting host resistance. These results are an important step toward identification of genes underlying resistance to OsHV in oyster, and a step toward applying genomic data to enhance selective breeding for disease resistance in oyster aquaculture.
Avainsanat: GWAS
OsHV-1
SNP array
linkage map
oysters
MICROSATELLITE DNA MARKERS
SINGLE NUCLEOTIDE POLYMORPHISMS
LINKAGE MAPS
MORTALITY
VIRUS
DISEASE
FRANCE
ERRORS
QTL
1184 Genetics, developmental biology, physiology
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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