MKLN1 splicing defect in dogs with lethal acrodermatitis

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Bauer , A , Jagannathan , V , Hoegler , S , Richter , B , McEwan , N A , Thomas , A , Cadieu , E , Andre , C , Hytonen , M K , Lohi , H , Welle , M M , Roosje , P , Mellersh , C , Casal , M L & Leeb , T 2018 , ' MKLN1 splicing defect in dogs with lethal acrodermatitis ' , PLoS Genetics , vol. 14 , no. 3 , 1007264 .

Title: MKLN1 splicing defect in dogs with lethal acrodermatitis
Author: Bauer, Anina; Jagannathan, Vidhya; Hoegler, Sandra; Richter, Barbara; McEwan, Neil A.; Thomas, Anne; Cadieu, Edouard; Andre, Catherine; Hytonen, Marjo K.; Lohi, Hannes; Welle, Monika M.; Roosje, Petra; Mellersh, Cathryn; Casal, Margret L.; Leeb, Tosso
Contributor: University of Helsinki, Research Programs Unit
University of Helsinki, Medicum
Date: 2018-03
Language: eng
Number of pages: 14
Belongs to series: PLoS Genetics
ISSN: 1553-7404
Abstract: Lethal acrodermatitis (LAD) is a genodermatosis with monogenic autosomal recessive inheritance in Bull Terriers and Miniature Bull Terriers. The LAD phenotype is characterized by poor growth, immune deficiency, and skin lesions, especially at the paws. Utilizing a combination of genome wide association study and haplotype analysis, we mapped the LAD locus to a critical interval of similar to 1.11 Mb on chromosome 14. Whole genome sequencing of an LAD affected dog revealed a splice region variant in the MKLN1 gene that was not present in 191 control genomes (chr14:5,731,405T>G or MKLN/:c.400+3A>C). This variant showed perfect association in a larger combined Bull Terrier/Miniature Bull Terrier cohort of 46 cases and 294 controls. The variant was absent from 462 genetically diverse control dogs of 62 other dog breeds. RT-PCR analysis of skin RNA from an affected and a control dog demonstrated skipping of exon 4 in the MKLN1 transcripts of the LAD affected dog, which leads to a shift in the MKLN1 reading frame. MKLN1 encodes the widely expressed intracellular protein muskelin 1, for which diverse functions in cell adhesion, morphology, spreading, and intracellular transport processes are discussed. While the pathogenesis of LAD remains unclear, our data facilitate genetic testing of Bull Terriers and Miniature Bull Terriers to prevent the unintentional production of LAD affected dogs. This study may provide a starting point to further clarify the elusive physiological role of muskelin 1 in vivo.
1184 Genetics, developmental biology, physiology
413 Veterinary science

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