Mesenchymal Stromal Cells and Their Extracellular Vesicles Enhance the Anti-Inflammatory Phenotype of Regulatory Macrophages by Downregulating the Production of Interleukin (IL)-23 and IL-22

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Hyvärinen , K , Holopainen , M , Skirdenko , V , Ruhanen , H , Lehenkari , P , Korhonen , M , Käkelä , R , Laitinen , S & Kerkelä , E 2018 , ' Mesenchymal Stromal Cells and Their Extracellular Vesicles Enhance the Anti-Inflammatory Phenotype of Regulatory Macrophages by Downregulating the Production of Interleukin (IL)-23 and IL-22 ' , Frontiers in Immunology , vol. 9 , 771 . https://doi.org/10.3389/fimmu.2018.00771

Title: Mesenchymal Stromal Cells and Their Extracellular Vesicles Enhance the Anti-Inflammatory Phenotype of Regulatory Macrophages by Downregulating the Production of Interleukin (IL)-23 and IL-22
Author: Hyvärinen, Kati; Holopainen, Minna; Skirdenko, Vita; Ruhanen, Hanna; Lehenkari, Petri; Korhonen, Matti; Käkelä, Reijo; Laitinen, Saara; Kerkelä, Erja
Contributor: University of Helsinki, Finnish Red Cross Blood Service
University of Helsinki, Biosciences
University of Helsinki, Molecular and Integrative Biosciences Research Programme
Date: 2018-04-12
Language: eng
Number of pages: 13
Belongs to series: Frontiers in Immunology
ISSN: 1664-3224
URI: http://hdl.handle.net/10138/234656
Abstract: Resolution-phase macrophage population orchestrates active dampening of the inflammation by secreting anti-inflammatory and proresolving products including interleukin (IL)-10 and lipid mediators (LMs). We investigated the effects of both human bone marrow-derived mesenchymal stromal cells (MSCs) and MSC-derived extracellular vesicles (MSC-EVs) on mature human regulatory macrophages (Mregs). The cytokines and LMs were determined from cell culture media of Mregs cultivated with MSCs and MSC-EVs. In addition, the alterations in the expression of cell surface markers and the phagocytic ability of Mregs were investigated. Our novel findings indicate that both MSC coculture and MSC-EVs downregulated the production of IL-23 and IL-22 enhancing the anti-inflammatory phenotype of Mregs and amplifying proresolving properties. The levels of prostaglandin E-2 (PGE(2)) were substantially upregulated in MSC coculture media, which may endorse proresolving LM class switching. In addition, our results manifest, for the first time, that MSC-EVs mediate the Mreg phenotype change via PGE(2). These data suggest that both human MSC and MSC-EVs may potentiate tolerance-promoting proresolving phenotype of human Mregs.
Subject: regulatory macrophages
mesenchymal stromal cells
extracellular vesicles
interleukin-23
prostaglandin E-2
resolution
STEM-CELLS
T-CELLS
THERAPEUTIC TARGETS
IN-VITRO
RESOLUTION
INFLAMMATION
RESPONSES
INDUCE
PHASE
ACTIVATION
3121 General medicine, internal medicine and other clinical medicine
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