Prevention of Biomaterial Infection by Pre-Operative Incubation with Human Cells

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Pérez Tanoira , R , Aarnisalo , A A , Eklund , K , Han , X , Soininen , A , Tiainen , V-M , Esteban , J & Kinnari , T J 2017 , ' Prevention of Biomaterial Infection by Pre-Operative Incubation with Human Cells ' , Surgical Infections , vol. 18 , no. 3 , pp. 336-344 .

Title: Prevention of Biomaterial Infection by Pre-Operative Incubation with Human Cells
Author: Pérez Tanoira, Ramón; Aarnisalo, Antti A.; Eklund, Kari; Han, Xia; Soininen, Antti; Tiainen, Veli-Matti; Esteban, Jaime; Kinnari, Teemu J.
Contributor organization: Clinicum
Korva-, nenä- ja kurkkutautien klinikka
Department of Ophthalmology and Otorhinolaryngology
Reumatologian yksikkö
Department of Medicine
HUS Head and Neck Center
HUS Inflammation Center
Date: 2017-04
Language: eng
Number of pages: 9
Belongs to series: Surgical Infections
ISSN: 1096-2964
Abstract: Background: Cells of tissues and biofilm forming bacteria compete for the living space on the surface of an implant. We hypothesized the incubation of the implant (titanium, polydimethylsiloxane, and polystyrene surface) with human cells before implantation as a strategy to prevent bacterial adhesion and biofilm formation. Methods: After 24 hours of incubation with human osteogenic sarcoma SaOS-2 cells (1x10(5) cells/mL), the materials were incubated for 4.5 hours or two days with Staphylococcus aureus in serial 1:10 dilutions of 10(8) colony-forming units/mL. The bacterial adherence and biofilm biomass on materials pre-incubated with SaOS-2 cells were compared with our previous results on materials incubated only with bacteria or in simultaneous co-culture of SaOS-2 cells and S. aureus. Fluorescent microscopy and crystal violet stain were used. The number of viable SaOS-2 and bacterial cells present was tested using colorimetric methods (MTT, LDH) and drop plate method, respectively. Results: The pre-treatment with human cells was associated with a reduction of bacterial colonization of the biomaterial at 4.5 hours and 48 hours compared with the non-pre-treated materials. The presence of bacteria decreased the number of viable human cells on all materials. (Supplementary Fig. 1; see online supplementary materials at Conclusions: These results suggest that the pre-operative incubation of prostheses with host cells could prevent infection of biomaterials.
Subject: bacterial adherence
pre-operative incubation
race for the surface
3126 Surgery, anesthesiology, intensive care, radiology
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Rights: unspecified
Usage restriction: openAccess
Self-archived version: publishedVersion

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