Prevention of Biomaterial Infection by Pre-Operative Incubation with Human Cells

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http://hdl.handle.net/10138/234687

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Pérez Tanoira , R , Aarnisalo , A A , Eklund , K , Han , X , Soininen , A , Tiainen , V-M , Esteban , J & Kinnari , T J 2017 , ' Prevention of Biomaterial Infection by Pre-Operative Incubation with Human Cells ' , Surgical Infections , vol. 18 , no. 3 , pp. 336-344 . https://doi.org/10.1089/sur.2016.263

Title: Prevention of Biomaterial Infection by Pre-Operative Incubation with Human Cells
Author: Pérez Tanoira, Ramón; Aarnisalo, Antti A.; Eklund, Kari; Han, Xia; Soininen, Antti; Tiainen, Veli-Matti; Esteban, Jaime; Kinnari, Teemu J.
Contributor: University of Helsinki, Clinicum
University of Helsinki, Korva-, nenä- ja kurkkutautien klinikka
University of Helsinki, Clinicum
University of Helsinki, Clinicum
Date: 2017-04
Language: eng
Number of pages: 9
Belongs to series: Surgical Infections
ISSN: 1096-2964
URI: http://hdl.handle.net/10138/234687
Abstract: Background: Cells of tissues and biofilm forming bacteria compete for the living space on the surface of an implant. We hypothesized the incubation of the implant (titanium, polydimethylsiloxane, and polystyrene surface) with human cells before implantation as a strategy to prevent bacterial adhesion and biofilm formation. Methods: After 24 hours of incubation with human osteogenic sarcoma SaOS-2 cells (1x10(5) cells/mL), the materials were incubated for 4.5 hours or two days with Staphylococcus aureus in serial 1:10 dilutions of 10(8) colony-forming units/mL. The bacterial adherence and biofilm biomass on materials pre-incubated with SaOS-2 cells were compared with our previous results on materials incubated only with bacteria or in simultaneous co-culture of SaOS-2 cells and S. aureus. Fluorescent microscopy and crystal violet stain were used. The number of viable SaOS-2 and bacterial cells present was tested using colorimetric methods (MTT, LDH) and drop plate method, respectively. Results: The pre-treatment with human cells was associated with a reduction of bacterial colonization of the biomaterial at 4.5 hours and 48 hours compared with the non-pre-treated materials. The presence of bacteria decreased the number of viable human cells on all materials. (Supplementary Fig. 1; see online supplementary materials at www.liebertpub.com/sur). Conclusions: These results suggest that the pre-operative incubation of prostheses with host cells could prevent infection of biomaterials.
Subject: bacterial adherence
biofilm
biomaterial
pre-operative incubation
race for the surface
ORTHOPEDIC IMPLANTS
BIOFILM FORMATION
STAPHYLOCOCCUS-AUREUS
ANTIBIOTIC-RESISTANCE
TISSUE INTEGRATION
ADHESION
SURFACES
EPIDERMIDIS
BACTERIA
COATINGS
3126 Surgery, anesthesiology, intensive care, radiology
3121 General medicine, internal medicine and other clinical medicine
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