Differences in proliferation rate between CADASIL and control vascular smooth muscle cells are related to increased TGF beta expression

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dc.contributor.author Panahi, Mahmod
dc.contributor.author Mesri, Naeimeh Yousefi
dc.contributor.author Samuelsson, Eva-Britt
dc.contributor.author Coupland, Kirsten G.
dc.contributor.author Forsell, Charlotte
dc.contributor.author Graff, Caroline
dc.contributor.author Tikka, Saara
dc.contributor.author Winblad, Bengt
dc.contributor.author Viitanen, Matti
dc.contributor.author Karlström, Helena
dc.contributor.author Sundström, Erik
dc.contributor.author Behbahani, Homira
dc.date.accessioned 2018-06-25T06:35:01Z
dc.date.available 2018-06-25T06:35:01Z
dc.date.issued 2018-06
dc.identifier.citation Panahi , M , Mesri , N Y , Samuelsson , E-B , Coupland , K G , Forsell , C , Graff , C , Tikka , S , Winblad , B , Viitanen , M , Karlström , H , Sundström , E & Behbahani , H 2018 , ' Differences in proliferation rate between CADASIL and control vascular smooth muscle cells are related to increased TGF beta expression ' , Journal of Cellular and Molecular Medicine , vol. 22 , no. 6 , pp. 3016-3024 . https://doi.org/10.1111/jcmm.13534
dc.identifier.other PURE: 108181284
dc.identifier.other PURE UUID: 70aad941-94ca-4b1d-a141-fb4f5f970b44
dc.identifier.other WOS: 000433580300006
dc.identifier.other Scopus: 85043584528
dc.identifier.uri http://hdl.handle.net/10138/236615
dc.description.abstract Cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a familial fatal progressive degenerative disorder. One of the pathological hallmarks of CADASIL is a dramatic reduction of vascular smooth muscle cells (VSMCs) in cerebral arteries. Using VSMCs from the vasculature of the human umbilical cord, placenta and cerebrum of CADASIL patients, we found that CADASIL VSMCs had a lower proliferation rate compared to control VSMCs. Exposure of control VSMCs and endothelial cells (ECs) to media derived from CADASIL VSMCs lowered the proliferation rate of all cells examined. By quantitative RT-PCR analysis, we observed increased Transforming growth factor-beta (TGF beta) gene expression in CADASIL VSMCs. Adding TGF beta-neutralizing antibody restored the proliferation rate of CADASIL VSMCs. We assessed proliferation differences in the presence or absence of TGF beta-neutralizing antibody in ECs co-cultured with VSMCs. ECs co-cultured with CADASIL VSMCs exhibited a lower proliferation rate than those co-cultured with control VSMCs, and neutralization of TGF beta normalized the proliferation rate of ECs co-cultured with CADASIL VSMCs. We suggest that increased TGF beta expression in CADASIL VSMCs is involved in the reduced VSMC proliferation in CADASIL and may play a role in situ in altered proliferation of neighbouring cells in the vasculature. en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof Journal of Cellular and Molecular Medicine
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject CADASIL
dc.subject endothelial cells
dc.subject NOTCH3
dc.subject Transforming growth factor-beta
dc.subject vascular smooth muscle cells
dc.subject WHITE-MATTER
dc.subject MUTATIONS
dc.subject SPECTRUM
dc.subject ENDOGLIN
dc.subject 3111 Biomedicine
dc.title Differences in proliferation rate between CADASIL and control vascular smooth muscle cells are related to increased TGF beta expression en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization Department of Biochemistry and Developmental Biology
dc.contributor.organization University of Helsinki
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1111/jcmm.13534
dc.relation.issn 1582-4934
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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