Genome-Wide Exon-Capture Approach Identifies Genetic Variants of Norway Spruce Genes Associated With Susceptibility to Heterobasidion parviporum Infection

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http://hdl.handle.net/10138/236808

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Mukrimin , M , Kovalchuk , A , Neves , L G , Jaber , E H A , Haapanen , M , Kirst , M & Asiegbu , F O 2018 , ' Genome-Wide Exon-Capture Approach Identifies Genetic Variants of Norway Spruce Genes Associated With Susceptibility to Heterobasidion parviporum Infection ' , Frontiers in plant science , vol. 9 , 793 . https://doi.org/10.3389/fpls.2018.00793

Julkaisun nimi: Genome-Wide Exon-Capture Approach Identifies Genetic Variants of Norway Spruce Genes Associated With Susceptibility to Heterobasidion parviporum Infection
Tekijä: Mukrimin, Mukrimin; Kovalchuk, Andriy; Neves, Leandro G.; Jaber, Emad H. A.; Haapanen, Matti; Kirst, Matias; Asiegbu, Fred O.
Tekijän organisaatio: Department of Forest Sciences
Frederick Asiegbu / Principal Investigator
Viikki Plant Science Centre (ViPS)
Helsinki Institute of Sustainability Science (HELSUS)
Forest Ecology and Management
Päiväys: 2018-06-12
Kieli: eng
Sivumäärä: 13
Kuuluu julkaisusarjaan: Frontiers in plant science
ISSN: 1664-462X
DOI-tunniste: https://doi.org/10.3389/fpls.2018.00793
URI: http://hdl.handle.net/10138/236808
Tiivistelmä: Root and butt rot caused by members of the Heterobasidion annosum species complex is the most economically important disease of conifer trees in boreal forests. Wood decay in the infected trees dramatically decreases their value and causes considerable losses to forest owners. Trees vary in their susceptibility to Heterobasidion infection, but the genetic determinants underlying the variation in the susceptibility are not well-understood. We performed the identification of Norway spruce genes associated with the resistance to Heterobasidion parviporum infection using genome-wide exon-capture approach. Sixty-four clonal Norway spruce lines were phenotyped, and their responses to H. parviporum inoculation were determined by lesion length measurements. Afterwards, the spruce lines were genotyped by targeted resequencing and identification of genetic variants (SNPs). Genome-wide association analysis identified 10 SNPs located within 8 genes as significantly associated with the larger necrotic lesions in response to H. parviporum inoculation. The genetic variants identified in our analysis are potential marker candidates for future screening programs aiming at the differentiation of disease-susceptible and resistant trees.
Avainsanat: exome resequencing
genome-wide association studies (GWAS)
Heterobasidion
root and butt rot
Norway spruce
single-nucleotide polymorphism (SNP)
PINE PINUS-SYLVESTRIS
PICEA-ABIES
MAPPING POPULATION
ANNOSUM
ROOT
CLONES
GROWTH
ARABIDOPSIS
CONIFER
PROTEIN
1183 Plant biology, microbiology, virology
4112 Forestry
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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