Screening of the two-component-system histidine kinases of Listeria monocytogenes EGD-e. LiaS is needed for growth under heat, acid, alkali, osmotic, ethanol and oxidative stresses

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Pöntinen , A , Lindström , M , Skurnik , M & Korkeala , H 2017 , ' Screening of the two-component-system histidine kinases of Listeria monocytogenes EGD-e. LiaS is needed for growth under heat, acid, alkali, osmotic, ethanol and oxidative stresses ' , Food Microbiology , vol. 65 , pp. 36-43 . https://doi.org/10.1016/j.fm.2017.01.018

Title: Screening of the two-component-system histidine kinases of Listeria monocytogenes EGD-e. LiaS is needed for growth under heat, acid, alkali, osmotic, ethanol and oxidative stresses
Author: Pöntinen, Anna; Lindström, Miia; Skurnik, Mikael; Korkeala, Hannu
Other contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Research Programs Unit
University of Helsinki, Departments of Faculty of Veterinary Medicine










Date: 2017-08
Language: eng
Number of pages: 8
Belongs to series: Food Microbiology
ISSN: 0740-0020
DOI: https://doi.org/10.1016/j.fm.2017.01.018
URI: http://hdl.handle.net/10138/236973
Abstract: To study the role of each two-component system (TCS) histidine kinase (HK) in stress tolerance of Listeria monocytogenes EGD-e, we monitored the growth of individual HIC deletion mutant strains under heat (42.5 degrees C), acid (pH 5.6), alkali (pH 9.4), osmotic (6% NaCl), ethanol (3.5 vol%), and oxidative (5 mM H2O2) stresses. The growth of Delta liaS (Delta lmo1021) strain was impaired under each stress, with the most notable decrease under heat and osmotic stresses. The Delta ivirS (Delta lmo1741) strain showed nearly completely restricted growth at high temperature and impaired growth in ethanol. The growth of Delta agrC (Delta lmo0050) strain was impaired under osmotic stress and slightly under oxidative stress. We successfully complemented the HIC mutations using a novel allelic exchange based approach. This approach avoided the copy-number problems associated with in trans complementation from a plasmid. The mutant phenotypes were restored to the wild-type level in the complemented strains. This study reveals novel knowledge on the HKs needed for growth of L monocytogenes EGD-e under abovementioned stress conditions, with LiaS playing multiple roles in stress tolerance of L monocytogenes EGD-e. (C) 2017 Elsevier Ltd. All rights reserved.
Subject: Listeria monocytogenes
Two-component system
Histidine kinase
Stress response
SIGNAL-TRANSDUCTION SYSTEM
RESPONSE REGULATOR
LOW-TEMPERATURES
VIRULENCE
IDENTIFICATION
LISRK
GENES
LOCUS
RESISTANCE
TOLERANCE
1183 Plant biology, microbiology, virology
416 Food Science
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