Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells

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http://hdl.handle.net/10138/237076

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Redchuk , T A , Karasev , M M , Omelina , E S & Verkhusha , V V 2018 , ' Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells ' , ChemBioChem : a European journal of chemical biology , vol. 19 , no. 12 , pp. 1334-1340 . https://doi.org/10.1002/cbic.201700642

Title: Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells
Author: Redchuk, Taras A.; Karasev, Maksim M.; Omelina, Evgeniya S.; Verkhusha, Vladislav V.
Contributor: University of Helsinki, Department of Anatomy
University of Helsinki, Doctoral Programme in Biomedicine
University of Helsinki, Department of Anatomy
University of Helsinki, Department of Anatomy
Date: 2018-06-18
Language: eng
Number of pages: 7
Belongs to series: ChemBioChem : a European journal of chemical biology
ISSN: 1439-4227
URI: http://hdl.handle.net/10138/237076
Abstract: Near-infrared (NIR) light-inducible binding of bacterial phytochrome BphP1 to its engineered partner, QPAS1, is used for optical protein regulation in mammalian cells. However, there are no data on the application of the BphP1-QPAS1 pair in cells derived from various mammalian tissues. Here, we tested the functionality of two BphP1-QPAS1-based optogenetic toolsan NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)in several cell types, including cortical neurons. We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS. In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types. The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells. The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Subject: optogenetic
BphP1
QPAS1
near-infrared
gene expression
FLUORESCENT PROTEINS
BACTERIAL PHYTOCHROMES
SPATIOTEMPORAL CONTROL
OPTOGENETIC TOOLS
NUCLEAR TRANSPORT
IMAGE-ANALYSIS
BIOSENSORS
SYSTEM
LOCALIZATION
TRANSFECTION
1182 Biochemistry, cell and molecular biology
3111 Biomedicine
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