Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries

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Gomes-Silva , W , Prado-Ribeiro , A C , Brandao , T B , Morais-Faria , K , de Castro Junior , G , Mak , M P , Lopes , M A , Rocha , M M , Salo , T , Tjaderhane , L , de Goes , M F & Santos-Silva , A R 2017 , ' Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries ' , Caries Research , vol. 51 , no. 3 , pp. 216-224 . https://doi.org/10.1159/000457806

Title: Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries
Author: Gomes-Silva, Wagner; Prado-Ribeiro, Ana Carolina; Brandao, Thais Bianca; Morais-Faria, Karina; de Castro Junior, Gilberto; Mak, Milena Perez; Lopes, Marcio Ajudarte; Rocha, Marcelo Marques; Salo, Tuula; Tjaderhane, Leo; de Goes, Mario Fernando; Santos-Silva, Alan Roger
Contributor: University of Helsinki, Clinicum
University of Helsinki, Clinicum
Date: 2017
Language: eng
Number of pages: 9
Belongs to series: Caries Research
ISSN: 0008-6568
URI: http://hdl.handle.net/10138/237096
Abstract: Recent evidence suggests that head-and-neck radiotherapy (HNRT) increases active forms of matrix metalloproteinase-20 (MMP-20) in human tooth crowns, degrading the dentin-enamel junction (DEJ) and leading to enamel delamination, which is a pivotal step in the formation of radiation-related caries (RRC). Additional participation of enzymatic degradation of organic matrix components in caries progression was attributed to MMP-20 in dentin. Therefore, the current study tested the hypothesis that MMP-20 is overexpressed in the DEJ, dentin-pulp complex components, and carious dentin of post-HNRT patients, leading to detectable micromorphological changes to the enamel and dentin. Thirty-six teeth were studied, including 19 post-HNRT specimens and 17 nonirradiated controls. Optical light microscopy was used to investigate the micromorphological components of the DEJ, dentin-pulp complex components, and carious dentin. The samples were divided into 2 subgroups: nondemineralized ground sections (n = 20) and demineralized histological sections (n = 16). In addition, immunohistochemical analysis using the immunoperoxidase technique was conducted to semiquantitatively assess MMP-20 expression in the DEJ, dentin-pulp complex components, and carious dentin. No apparent damage to the DEJ microstructure or other dentin-pulp complex components was observed and no statistically significant differences were detected in MMP-20 expression (p > 0.05) between the irradiated and control groups. This study rejected the hypothesis that MMP-20 is overexpressed in the DEJ, dentin-pulp complex components, and carious dentin of post-HNRT patients, leading to detectable micromorphological changes. Hence, direct effects of radiation may not be regarded as an independent factor to explain aggressive clinical patterns of RRC. (C) 2017 S. Karger AG, Basel
Subject: Caries
Dental tissues
Dentin-enamel junction
Head-and-neck cancer
Matrix metalloproteinase-20
Radiotherapy
HOST-DERIVED PROTEINASES
ORAL COMPLICATIONS
CANCER-PATIENTS
NECK-CANCER
RADIOTHERAPY
COLLAGEN
MMP-20
HEAD
ENAMELYSIN
TEETH
313 Dentistry
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