Environmental properties of cells improve machine learning-based phenotype recognition accuracy

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http://hdl.handle.net/10138/237209

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Toth , T , Balassa , T , Bara , N , Kovacs , F , Kriston , A , Molnar , C , Haracska , L , Sukosd , F & Horvath , P 2018 , ' Environmental properties of cells improve machine learning-based phenotype recognition accuracy ' , Scientific Reports , vol. 8 , 10085 . https://doi.org/10.1038/s41598-018-28482-y

Title: Environmental properties of cells improve machine learning-based phenotype recognition accuracy
Author: Toth, Timea; Balassa, Tamas; Bara, Norbert; Kovacs, Ferenc; Kriston, Andras; Molnar, Csaba; Haracska, Lajos; Sukosd, Farkas; Horvath, Peter
Other contributor: University of Helsinki, Institute for Molecular Medicine Finland

Date: 2018-07-04
Language: eng
Number of pages: 9
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-018-28482-y
URI: http://hdl.handle.net/10138/237209
Abstract: To answer major questions of cell biology, it is often essential to understand the complex phenotypic composition of cellular systems precisely. Modern automated microscopes produce vast amounts of images routinely, making manual analysis nearly impossible. Due to their efficiency, machine learningbased analysis software have become essential tools to perform single-cell-level phenotypic analysis of large imaging datasets. However, an important limitation of such methods is that they do not use the information gained from the cellular micro-and macroenvironment: the algorithmic decision is based solely on the local properties of the cell of interest. Here, we present how various features from the surrounding environment contribute to identifying a cell and how such additional information can improve single-cell-level phenotypic image analysis. The proposed methodology was tested for different sizes of Euclidean and nearest neighbour-based cellular environments both on tissue sections and cell cultures. Our experimental data verify that the surrounding area of a cell largely determines its entity. This effect was found to be especially strong for established tissues, while it was somewhat weaker in the case of cell cultures. Our analysis shows that combining local cellular features with the properties of the cell's neighbourhood significantly improves the accuracy of machine learning-based phenotyping.
Subject: STOCHASTIC GENE-EXPRESSION
HIGH-CONTENT SCREENS
IMAGE-BASED SCREENS
DATA EXPLORATION
MICROSCOPY
CLASSIFICATION
CELLCLASSIFIER
VARIABILITY
SOFTWARE
SETS
3111 Biomedicine
1184 Genetics, developmental biology, physiology
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