A high-throughput method for orthophosphate determination of thermostable membrane-bound pyrophosphatase activity

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dc.contributor.author Vidilaseris, Keni
dc.contributor.author Kellosalo, Juho
dc.contributor.author Goldman, Adrian
dc.date.accessioned 2018-07-23T09:32:01Z
dc.date.available 2018-07-23T09:32:01Z
dc.date.issued 2018-02-14
dc.identifier.citation Vidilaseris , K , Kellosalo , J & Goldman , A 2018 , ' A high-throughput method for orthophosphate determination of thermostable membrane-bound pyrophosphatase activity ' , Analytical Methods , vol. 10 , no. 6 , pp. 646-651 . https://doi.org/10.1039/c7ay02558k
dc.identifier.other PURE: 102157738
dc.identifier.other PURE UUID: 8c10f04a-4e5c-43c5-a5a3-a44cfd81da37
dc.identifier.other WOS: 000424767800011
dc.identifier.other Scopus: 85041734605
dc.identifier.other ORCID: /0000-0001-8032-9700/work/54147003
dc.identifier.uri http://hdl.handle.net/10138/237234
dc.description.abstract Membrane-bound pyrophosphatases (mPPases) are homodimeric integral membrane proteins that hydrolyse pyrophosphate into orthophosphates coupled to the active transport of protons or sodium ions across membranes. They occur in bacteria, archaea, plants, and protist parasites. As they are essential in protist parasites and there are no homologous proteins in animals and humans, these enzymes represent an excellent drug target for treating protistal diseases. Experimental screening to find drug candidates is an important step to discover new hit compounds. For that, a cheap, simple, and robust assay is needed. Here we report the application of the molybdenum blue reaction method for a medium throughput microplate activity assay of the hyperthermophilic bacterium Thermotoga maritima mPPase and the possible application of the assay to screen inhibitors of membrane-bound pyrophosphatases. en
dc.format.extent 6
dc.language.iso eng
dc.relation.ispartof Analytical Methods
dc.rights cc_by_nc
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject LABILE ORGANIC PHOSPHATE
dc.subject COLI INORGANIC PYROPHOSPHATASE
dc.subject COLORIMETRIC DETERMINATION
dc.subject THERMOTOGA-MARITIMA
dc.subject HETEROLOGOUS EXPRESSION
dc.subject PUMPING PYROPHOSPHATASE
dc.subject CRYSTAL-STRUCTURE
dc.subject ASSAY
dc.subject ACIDOCALCISOMES
dc.subject PURIFICATION
dc.subject 116 Chemical sciences
dc.title A high-throughput method for orthophosphate determination of thermostable membrane-bound pyrophosphatase activity en
dc.type Article
dc.contributor.organization Biosciences
dc.contributor.organization Institute of Biotechnology
dc.contributor.organization Biochemistry and Biotechnology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1039/c7ay02558k
dc.relation.issn 1759-9660
dc.rights.accesslevel openAccess

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