Quad-mode functional and molecular photoacoustic microscopy

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http://hdl.handle.net/10138/237860

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Liu , W , Shcherbakova , D M , Kurupassery , N , Li , Y , Zhou , Q , Verkhusha , V V & Yao , J 2018 , ' Quad-mode functional and molecular photoacoustic microscopy ' , Scientific Reports , vol. 8 , 11123 . https://doi.org/10.1038/s41598-018-29249-1

Title: Quad-mode functional and molecular photoacoustic microscopy
Author: Liu, Wei; Shcherbakova, Daria M.; Kurupassery, Neel; Li, Yang; Zhou, Qifa; Verkhusha, Vladislav V.; Yao, Junjie
Other contributor: University of Helsinki, Medicum


Date: 2018-07-24
Language: eng
Number of pages: 10
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-018-29249-1
URI: http://hdl.handle.net/10138/237860
Abstract: A conventional photoacoustic microscopy (PAM) system typically has to make tradeoffs between its spatial resolution and penetration depth, by choosing a fixed configuration of optical excitation and acoustic detection. The single-scale imaging capability of PAM may limit its applications in biomedical studies. Here, we report a quad-mode photoacoustic microscopy (QM-PAM) system with four complementary spatial resolutions and maximum penetration depths. For this we first developed a ring-shaped focused ultrasound transducer that has two independent elements with respective central frequencies at 20 MHz and 40 MHz, providing complementary acoustically-determined spatial resolutions and penetration depths. To accommodate the dual-element ultrasound transducer, we implemented two optical excitation modes to provide tightly-and weakly-focused light illumination. The dual-element acoustic detection combined with the two optical focusing modes can thus provide four imaging scales in a single imaging device, with consistent contrast mechanisms and co-registered field of views. We have demonstrated the multiscale morphological, functional, and molecular imaging capability of QM-PAM in the mouse head, leg and ear in vivo. We expect the high scale flexibility of QM-PAM will enable broad applications in preclinical studies.
Subject: INFRARED FLUORESCENT PROTEINS
SPECKLE-FREE NATURE
HIGH-RESOLUTION
MOUSE-BRAIN
TOMOGRAPHY
PHYTOCHROMES
BIOSENSORS
CANCER
3111 Biomedicine
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