Quad-mode functional and molecular photoacoustic microscopy

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dc.contributor.author Liu, Wei
dc.contributor.author Shcherbakova, Daria M.
dc.contributor.author Kurupassery, Neel
dc.contributor.author Li, Yang
dc.contributor.author Zhou, Qifa
dc.contributor.author Verkhusha, Vladislav V.
dc.contributor.author Yao, Junjie
dc.date.accessioned 2018-08-08T11:23:01Z
dc.date.available 2018-08-08T11:23:01Z
dc.date.issued 2018-07-24
dc.identifier.citation Liu , W , Shcherbakova , D M , Kurupassery , N , Li , Y , Zhou , Q , Verkhusha , V V & Yao , J 2018 , ' Quad-mode functional and molecular photoacoustic microscopy ' , Scientific Reports , vol. 8 , 11123 . https://doi.org/10.1038/s41598-018-29249-1
dc.identifier.other PURE: 114997357
dc.identifier.other PURE UUID: f77ad945-2a7d-48eb-8c04-be83af097266
dc.identifier.other WOS: 000439549800002
dc.identifier.other Scopus: 85050611244
dc.identifier.uri http://hdl.handle.net/10138/237860
dc.description.abstract A conventional photoacoustic microscopy (PAM) system typically has to make tradeoffs between its spatial resolution and penetration depth, by choosing a fixed configuration of optical excitation and acoustic detection. The single-scale imaging capability of PAM may limit its applications in biomedical studies. Here, we report a quad-mode photoacoustic microscopy (QM-PAM) system with four complementary spatial resolutions and maximum penetration depths. For this we first developed a ring-shaped focused ultrasound transducer that has two independent elements with respective central frequencies at 20 MHz and 40 MHz, providing complementary acoustically-determined spatial resolutions and penetration depths. To accommodate the dual-element ultrasound transducer, we implemented two optical excitation modes to provide tightly-and weakly-focused light illumination. The dual-element acoustic detection combined with the two optical focusing modes can thus provide four imaging scales in a single imaging device, with consistent contrast mechanisms and co-registered field of views. We have demonstrated the multiscale morphological, functional, and molecular imaging capability of QM-PAM in the mouse head, leg and ear in vivo. We expect the high scale flexibility of QM-PAM will enable broad applications in preclinical studies. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject INFRARED FLUORESCENT PROTEINS
dc.subject SPECKLE-FREE NATURE
dc.subject HIGH-RESOLUTION
dc.subject MOUSE-BRAIN
dc.subject TOMOGRAPHY
dc.subject PHYTOCHROMES
dc.subject BIOSENSORS
dc.subject CANCER
dc.subject 3111 Biomedicine
dc.title Quad-mode functional and molecular photoacoustic microscopy en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization University of Helsinki
dc.contributor.organization Faculty of Medicine
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1038/s41598-018-29249-1
dc.relation.issn 2045-2322
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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