In situ production of active vitamin B12 in cereal matrices using Propionibacterium freudenreichii

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Chamlagain , B , Sugito , T A , Deptula , P , Edelmann , M , Kariluoto , S , Varmanen , P & Piironen , V 2018 , ' In situ production of active vitamin B12 in cereal matrices using Propionibacterium freudenreichii ' , Food Science & Nutrition , vol. 6 , no. 1 , pp. 67-76 . https://doi.org/10.1002/fsn3.528

Title: In situ production of active vitamin B12 in cereal matrices using Propionibacterium freudenreichii
Author: Chamlagain, Bhawani; Sugito, Tessa Ayuningtyas; Deptula, Paulina; Edelmann, Minnamari; Kariluoto, Susanna; Varmanen, Pekka; Piironen, Vieno
Contributor: University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
Date: 2018-01
Language: eng
Number of pages: 10
Belongs to series: Food Science & Nutrition
ISSN: 2048-7177
URI: http://hdl.handle.net/10138/237876
Abstract: The in situ production of active vitamin B12 was investigated in aqueous cereal-based matrices with three strains of food-grade Propionibacterium freudenreichii. Matrices prepared from malted barley flour (33% w/v; BM), barley flour (6%; BF), and wheat aleurone (15%; AM) were fermented. The effect of cobalt and the lower ligand 5,6-dimethylbenzimidazole (DMBI) or its natural precursors (riboflavin and nicotinamide) on active B12 production was evaluated. Active B12 production was confirmed by UHPLC-UV-MS analysis. A B12 content of 12-37 mu g.kg(-1) was produced in BM; this content increased 10-fold with cobalt and reached 940-1,480 mu g.kg(-1) with both cobalt and DMBI. With riboflavin and nicotinamide, B12 production in cobalt-supplemented BM increased to 712 mu g.kg(-1). Approximately, 10 mu g.kg(-1) was achieved in BF and AM and was increased to 80 mu g.kg(-1) in BF and 260 mu g.kg(-1) in AM with cobalt and DMBI. The UHPLC and microbiological assay (MBA) results agreed when both cobalt and DMBI or riboflavin and nicotinamide were supplemented. However, MBA gave ca. 20%-40% higher results in BM and AM supplemented with cobalt, indicating the presence of human inactive analogues, such as pseudovitamin B12. This study demonstrates that cereal products can be naturally fortified with active B12 to a nutritionally relevant level by fermenting with P. freudenreichii.
Subject: 416 Food Science
barley malt and flour
fermentation
Propionibacterium freudenreichii
vitamin B12
wheat aleurone
LACTOBACILLUS-REUTERI
BIOSYNTHESIS
TEMPE
FERMENTATION
RIBOFLAVIN
DEFICIENCY
BACTERIA
CULTURES
5,6-DIMETHYLBENZIMIDAZOLE
BIOAVAILABILITY
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