Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo

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http://hdl.handle.net/10138/237993

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Li , L , Shemetov , A A , Baloban , M , Hu , P , Zhu , L , Shcherbakova , D M , Zhang , R , Shi , J , Yao , J , Wang , L V & Verkhusha , V V 2018 , ' Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo ' , Nature Communications , vol. 9 , 2734 . https://doi.org/10.1038/s41467-018-05231-3

Title: Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo
Author: Li, Lei; Shemetov, Anton A.; Baloban, Mikhail; Hu, Peng; Zhu, Liren; Shcherbakova, Daria M.; Zhang, Ruiying; Shi, Junhui; Yao, Junjie; Wang, Lihong V.; Verkhusha, Vladislav V.
Other contributor: University of Helsinki, Medicum

Date: 2018-07-16
Language: eng
Number of pages: 14
Belongs to series: Nature Communications
ISSN: 2041-1723
DOI: https://doi.org/10.1038/s41467-018-05231-3
URI: http://hdl.handle.net/10138/237993
Abstract: Photoacoustic (PA) computed tomography (PACT) benefits from genetically encoded probes with photochromic behavior, which dramatically increase detection sensitivity and specificity through photoswitching and differential imaging. Starting with a DrBphP bacterial phytochrome, we have engineered a near-infrared photochromic probe, DrBphP-PCM, which is superior to the full-length RpBphP1 phytochrome previously used in differential PACT. DrBphP-PCM has a smaller size, better folding, and higher photoswitching contrast. We have imaged both DrBphP-PCM and RpBphP1 simultaneously on the basis of their unique signal decay characteristics, using a reversibly switchable single-impulse panoramic PACT (RS-SIP-PACT) with a single wavelength excitation. The simple structural organization of DrBphPPCM allows engineering a bimolecular PA complementation reporter, a split version of DrBphP-PCM, termed DrSplit. DrSplit enables PA detection of protein-protein interactions in deep-seated mouse tumors and livers, achieving 125-mu m spatial resolution and 530-cell sensitivity in vivo. The combination of RS-SIP-PACT with DrBphP-PCM and DrSplit holds great potential for noninvasive multi-contrast deep-tissue functional imaging.
Subject: MULTISPECTRAL OPTOACOUSTIC TOMOGRAPHY
BIMOLECULAR FLUORESCENCE COMPLEMENTATION
OPTOGENETIC TOOLS
BACTERIOPHYTOCHROME
PHYTOCHROMES
BIOSENSORS
REPORTER
MICROSCOPY
CELLS
3111 Biomedicine
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