Li , L , Shemetov , A A , Baloban , M , Hu , P , Zhu , L , Shcherbakova , D M , Zhang , R , Shi , J , Yao , J , Wang , L V & Verkhusha , V V 2018 , ' Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo ' , Nature Communications , vol. 9 , 2734 . https://doi.org/10.1038/s41467-018-05231-3
Title: | Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo |
Author: | Li, Lei; Shemetov, Anton A.; Baloban, Mikhail; Hu, Peng; Zhu, Liren; Shcherbakova, Daria M.; Zhang, Ruiying; Shi, Junhui; Yao, Junjie; Wang, Lihong V.; Verkhusha, Vladislav V. |
Contributor organization: | Medicum University of Helsinki |
Date: | 2018-07-16 |
Language: | eng |
Number of pages: | 14 |
Belongs to series: | Nature Communications |
ISSN: | 2041-1723 |
DOI: | https://doi.org/10.1038/s41467-018-05231-3 |
URI: | http://hdl.handle.net/10138/237993 |
Abstract: | Photoacoustic (PA) computed tomography (PACT) benefits from genetically encoded probes with photochromic behavior, which dramatically increase detection sensitivity and specificity through photoswitching and differential imaging. Starting with a DrBphP bacterial phytochrome, we have engineered a near-infrared photochromic probe, DrBphP-PCM, which is superior to the full-length RpBphP1 phytochrome previously used in differential PACT. DrBphP-PCM has a smaller size, better folding, and higher photoswitching contrast. We have imaged both DrBphP-PCM and RpBphP1 simultaneously on the basis of their unique signal decay characteristics, using a reversibly switchable single-impulse panoramic PACT (RS-SIP-PACT) with a single wavelength excitation. The simple structural organization of DrBphPPCM allows engineering a bimolecular PA complementation reporter, a split version of DrBphP-PCM, termed DrSplit. DrSplit enables PA detection of protein-protein interactions in deep-seated mouse tumors and livers, achieving 125-mu m spatial resolution and 530-cell sensitivity in vivo. The combination of RS-SIP-PACT with DrBphP-PCM and DrSplit holds great potential for noninvasive multi-contrast deep-tissue functional imaging. |
Subject: |
MULTISPECTRAL OPTOACOUSTIC TOMOGRAPHY
BIMOLECULAR FLUORESCENCE COMPLEMENTATION OPTOGENETIC TOOLS BACTERIOPHYTOCHROME PHYTOCHROMES BIOSENSORS REPORTER MICROSCOPY CELLS 3111 Biomedicine |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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