Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production

Show full item record



Permalink

http://hdl.handle.net/10138/238496

Citation

Amini , P , Stojkov , D , Felser , A , Jackson , C B , Courage , C , Schaller , A , Gelman , L , Soriano , M E , Nuoffer , J-M , Scorrano , L , Benarafa , C , Yousefi , S & Simon , H-U 2018 , ' Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production ' , Nature Communications , vol. 9 , 2958 . https://doi.org/10.1038/s41467-018-05387-y

Title: Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production
Author: Amini, Poorya; Stojkov, Darko; Felser, Andrea; Jackson, Christopher B.; Courage, Carolina; Schaller, Andre; Gelman, Laurent; Soriano, Maria Eugenia; Nuoffer, Jean-Marc; Scorrano, Luca; Benarafa, Charaf; Yousefi, Shida; Simon, Hans-Uwe
Other contributor: University of Helsinki, Anu Wartiovaara / Principal Investigator



Date: 2018-07-27
Language: eng
Number of pages: 16
Belongs to series: Nature Communications
ISSN: 2041-1723
DOI: https://doi.org/10.1038/s41467-018-05387-y
URI: http://hdl.handle.net/10138/238496
Abstract: Optic atrophy 1 (OPA1) is a mitochondrial inner membrane protein that has an important role in mitochondrial fusion and structural integrity. Dysfunctional OPA1 mutations cause atrophy of the optic nerve leading to blindness. Here, we show that OPA1 has an important role in the innate immune system. Using conditional knockout mice lacking Opa1 in neutrophils (Opa1(N Delta)), we report that lack of OPA1 reduces the activity of mitochondrial electron transport complex I in neutrophils. This then causes a decline in adenosine-triphosphate (ATP) production through glycolysis due to lowered NAD(+) availability. Additionally, we show that OPA1-dependent ATP production in these cells is required for microtubule network assembly and for the formation of neutrophil extracellular traps. Finally, we show that Opa1(N Delta) mice exhibit a reduced antibacterial defense capability against Pseudomonas aeruginosa.
Subject: DOMINANT OPTIC ATROPHY
MITOCHONDRIAL-DNA
OPA1 MUTATIONS
BACTERIAL CLEARANCE
SKELETAL-MUSCLE
NADPH OXIDASE
FUSION
RELEASE
DEGRANULATION
METABOLISM
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
s41467_018_05387_y.pdf 4.102Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record