Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production

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dc.contributor.author Amini, Poorya
dc.contributor.author Stojkov, Darko
dc.contributor.author Felser, Andrea
dc.contributor.author Jackson, Christopher B.
dc.contributor.author Courage, Carolina
dc.contributor.author Schaller, Andre
dc.contributor.author Gelman, Laurent
dc.contributor.author Soriano, Maria Eugenia
dc.contributor.author Nuoffer, Jean-Marc
dc.contributor.author Scorrano, Luca
dc.contributor.author Benarafa, Charaf
dc.contributor.author Yousefi, Shida
dc.contributor.author Simon, Hans-Uwe
dc.date.accessioned 2018-08-16T08:50:01Z
dc.date.available 2018-08-16T08:50:01Z
dc.date.issued 2018-07-27
dc.identifier.citation Amini , P , Stojkov , D , Felser , A , Jackson , C B , Courage , C , Schaller , A , Gelman , L , Soriano , M E , Nuoffer , J-M , Scorrano , L , Benarafa , C , Yousefi , S & Simon , H-U 2018 , ' Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production ' , Nature Communications , vol. 9 , 2958 . https://doi.org/10.1038/s41467-018-05387-y
dc.identifier.other PURE: 115122431
dc.identifier.other PURE UUID: f359c3a3-6734-46db-8407-615ea7934025
dc.identifier.other WOS: 000439970700014
dc.identifier.other Scopus: 85050760532
dc.identifier.other ORCID: /0000-0003-1035-6417/work/67135419
dc.identifier.uri http://hdl.handle.net/10138/238496
dc.description.abstract Optic atrophy 1 (OPA1) is a mitochondrial inner membrane protein that has an important role in mitochondrial fusion and structural integrity. Dysfunctional OPA1 mutations cause atrophy of the optic nerve leading to blindness. Here, we show that OPA1 has an important role in the innate immune system. Using conditional knockout mice lacking Opa1 in neutrophils (Opa1(N Delta)), we report that lack of OPA1 reduces the activity of mitochondrial electron transport complex I in neutrophils. This then causes a decline in adenosine-triphosphate (ATP) production through glycolysis due to lowered NAD(+) availability. Additionally, we show that OPA1-dependent ATP production in these cells is required for microtubule network assembly and for the formation of neutrophil extracellular traps. Finally, we show that Opa1(N Delta) mice exhibit a reduced antibacterial defense capability against Pseudomonas aeruginosa. en
dc.format.extent 16
dc.language.iso eng
dc.relation.ispartof Nature Communications
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject DOMINANT OPTIC ATROPHY
dc.subject MITOCHONDRIAL-DNA
dc.subject OPA1 MUTATIONS
dc.subject BACTERIAL CLEARANCE
dc.subject SKELETAL-MUSCLE
dc.subject NADPH OXIDASE
dc.subject FUSION
dc.subject RELEASE
dc.subject DEGRANULATION
dc.subject METABOLISM
dc.subject 3111 Biomedicine
dc.title Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production en
dc.type Article
dc.contributor.organization Anu Wartiovaara / Principal Investigator
dc.contributor.organization Research Programme for Molecular Neurology
dc.contributor.organization Research Programs Unit
dc.contributor.organization University of Helsinki
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1038/s41467-018-05387-y
dc.relation.issn 2041-1723
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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