Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production

Show simple item record Amini, Poorya Stojkov, Darko Felser, Andrea Jackson, Christopher B. Courage, Carolina Schaller, Andre Gelman, Laurent Soriano, Maria Eugenia Nuoffer, Jean-Marc Scorrano, Luca Benarafa, Charaf Yousefi, Shida Simon, Hans-Uwe 2018-08-16T08:50:01Z 2018-08-16T08:50:01Z 2018-07-27
dc.identifier.citation Amini , P , Stojkov , D , Felser , A , Jackson , C B , Courage , C , Schaller , A , Gelman , L , Soriano , M E , Nuoffer , J-M , Scorrano , L , Benarafa , C , Yousefi , S & Simon , H-U 2018 , ' Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production ' , Nature Communications , vol. 9 , 2958 .
dc.identifier.other PURE: 115122431
dc.identifier.other PURE UUID: f359c3a3-6734-46db-8407-615ea7934025
dc.identifier.other WOS: 000439970700014
dc.identifier.other Scopus: 85050760532
dc.identifier.other ORCID: /0000-0003-1035-6417/work/67135419
dc.description.abstract Optic atrophy 1 (OPA1) is a mitochondrial inner membrane protein that has an important role in mitochondrial fusion and structural integrity. Dysfunctional OPA1 mutations cause atrophy of the optic nerve leading to blindness. Here, we show that OPA1 has an important role in the innate immune system. Using conditional knockout mice lacking Opa1 in neutrophils (Opa1(N Delta)), we report that lack of OPA1 reduces the activity of mitochondrial electron transport complex I in neutrophils. This then causes a decline in adenosine-triphosphate (ATP) production through glycolysis due to lowered NAD(+) availability. Additionally, we show that OPA1-dependent ATP production in these cells is required for microtubule network assembly and for the formation of neutrophil extracellular traps. Finally, we show that Opa1(N Delta) mice exhibit a reduced antibacterial defense capability against Pseudomonas aeruginosa. en
dc.format.extent 16
dc.language.iso eng
dc.relation.ispartof Nature Communications
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject OPA1 MUTATIONS
dc.subject NADPH OXIDASE
dc.subject FUSION
dc.subject RELEASE
dc.subject METABOLISM
dc.subject 3111 Biomedicine
dc.title Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production en
dc.type Article
dc.contributor.organization Anu Wartiovaara / Principal Investigator
dc.contributor.organization Research Programme for Molecular Neurology
dc.contributor.organization Research Programs Unit
dc.contributor.organization University of Helsinki
dc.description.reviewstatus Peer reviewed
dc.relation.issn 2041-1723
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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