The Synthetic Potential of Fungal Feruloyl Esterases : A Correlation with Current Classification Systems and Predicted Structural Properties

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Antonopoulou , I , Dilokpimol , A , Iancu , L , Mäkelä , M R , Varriale , S , Cerullo , G , Huttner , S , Uthoff , S , Juetten , P , Piechot , A , Steinbuechel , A , Olsson , L , Faraco , V , Hilden , K S , de Vries , R P , Rova , U & Christakopoulos , P 2018 , ' The Synthetic Potential of Fungal Feruloyl Esterases : A Correlation with Current Classification Systems and Predicted Structural Properties ' , Catalysts , vol. 8 , no. 6 , 242 . https://doi.org/10.3390/catal8060242

Title: The Synthetic Potential of Fungal Feruloyl Esterases : A Correlation with Current Classification Systems and Predicted Structural Properties
Author: Antonopoulou, Io; Dilokpimol, Adiphol; Iancu, Laura; Mäkelä, Miia R.; Varriale, Simona; Cerullo, Gabriella; Huttner, Silvia; Uthoff, Stefan; Juetten, Peter; Piechot, Alexander; Steinbuechel, Alexander; Olsson, Lisbeth; Faraco, Vincenza; Hilden, Kristiina S.; de Vries, Ronald P.; Rova, Ulrika; Christakopoulos, Paul
Contributor: University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
Date: 2018-06
Language: eng
Number of pages: 22
Belongs to series: Catalysts
ISSN: 2073-4344
URI: http://hdl.handle.net/10138/238894
Abstract: Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and l-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier l-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2, -6, -7 members) based on homology modeling and small molecular docking simulations.
Subject: transesterification
feruloyl esterase
antioxidants
phylogenetic classification
prenyl ferulate
prenyl caffeate
glyceryl ferulate
butyl ferulate
L-arabinose ferulate
sugar esters
ASPERGILLUS-NIGER
ENZYMATIC-SYNTHESIS
CATALYZED SYNTHESIS
FUSARIUM-OXYSPORUM
CRYSTAL-STRUCTURE
ACID ESTERASES
ESTERIFICATION
FAMILY
OLIGOSACCHARIDES
OPTIMIZATION
1183 Plant biology, microbiology, virology
116 Chemical sciences
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