Faba Bean (Vicia faba L.) Nodulating Rhizobia in Panxi, China, Are Diverse at Species, Plant Growth Promoting Ability, and Symbiosis Related Gene Levels

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Chen , Y X , Zou , L , Penttinen , P , Chen , Q , Li , Q Q , Wang , C Q & Xu , K W 2018 , ' Faba Bean (Vicia faba L.) Nodulating Rhizobia in Panxi, China, Are Diverse at Species, Plant Growth Promoting Ability, and Symbiosis Related Gene Levels ' , Frontiers in Microbiology , vol. 9 , 1338 . https://doi.org/10.3389/fmicb.2018.01338

Title: Faba Bean (Vicia faba L.) Nodulating Rhizobia in Panxi, China, Are Diverse at Species, Plant Growth Promoting Ability, and Symbiosis Related Gene Levels
Author: Chen, Yuan X.; Zou, Lan; Penttinen, Petri; Chen, Qiang; Li, Qi Q.; Wang, Chang Q.; Xu, Kai W.
Contributor: University of Helsinki, Environmental Sciences
Date: 2018-06-20
Language: eng
Number of pages: 10
Belongs to series: Frontiers in Microbiology
ISSN: 1664-302X
URI: http://hdl.handle.net/10138/239081
Abstract: We isolated 65 rhizobial strains from faba bean (Vicia faba L.) from Panxi, China, studied their plant growth promoting ability with nitrogen free hydroponics, genetic diversity with clustered analysis of combined ARDRA and IGS-RFLP, and phylogeny by sequence analyses of 16S rRNA gene, three housekeeping genes and symbiosis related genes. Eleven strains improved the plant shoot dry mass significantly comparing to that of not inoculated plants. According to the clustered analysis of combined ARDRA and IGS-RFLP the isolates were genetically diverse. Forty-one of 65 isolates represented Rhizobium anhuiense, and the others belonged to R. fabae, Rhizobium vallis, Rhizobium sophorae, Agrobacterium radiobacter, and four species related to Rhizobium and Agrobacterium. The isolates carried four and five genotypes of nifH and nodC, respectively, in six different nifH-nodC combinations. When looking at the species-nifH-nodC combinations it is noteworthy that all but two of the six R. anhuiense isolates were different. Our results suggested that faba bean rhizobia in Panxi are diverse at species, plant growth promoting ability and symbiosis related gene levels.
Subject: faba bean
rhizobia
genetic diversity
multilocus sequence analysis
symbiosis gene
lateral gene transfer
MULTILOCUS SEQUENCE-ANALYSIS
SP NOV.
LEUCAENA-LEUCOCEPHALA
PHASEOLUS-VULGARIS
ANALYSIS REVEALS
NODC
PHYLOGENY
NITROGEN
SICHUAN
LEGUME
1183 Plant biology, microbiology, virology
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