Single-molecule measurements of viral ssRNA packaging

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http://hdl.handle.net/10138/239660

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Hanhijarvi , K J , Ziedaite , G , Bamford , D H , Haeggstrom , E & Poranen , M M 2017 , ' Single-molecule measurements of viral ssRNA packaging ' , RNA , vol. 23 , no. 1 , pp. 119-129 . https://doi.org/10.1261/rna.057471.116

Titel: Single-molecule measurements of viral ssRNA packaging
Författare: Hanhijarvi, Kalle J.; Ziedaite, Gabija; Bamford, Dennis H.; Haeggstrom, Edward; Poranen, Minna M.
Upphovmannens organisation: Department of Physics
Biosciences
Institute of Biotechnology
Structure of the Viral Universe
Datum: 2017-01
Språk: eng
Sidantal: 11
Tillhör serie: RNA
ISSN: 1355-8382
DOI: https://doi.org/10.1261/rna.057471.116
Permanenta länken (URI): http://hdl.handle.net/10138/239660
Abstrakt: Genome packaging of double-stranded RNA (dsRNA) phages has been widely studied using biochemical and molecular biology methods. We adapted the existing in vitro packaging system of one such phage for single-molecule experimentation. To our knowledge, this is the first attempt to study the details of viral RNA packaging using optical tweezers. Pseudomonas phage phi 6 is a dsRNA virus with a tripartite genome. Positive-sense (+) single-stranded RNA (ssRNA) genome precursors are packaged into a preformed procapsid (PC), where negative strands are synthesized. We present single-molecule measurements of the viral ssRNA packaging by the phi 6 PC. Our data show that packaging proceeds intermittently in slow and fast phases, which likely reflects differences in the unfolding of the RNA secondary structures of the ssRNA being packaged. Although the mean packaging velocity was relatively low (0.07-0.54 nm/sec), packaging could reach 4.62 nm/sec during the fast packaging phase.
Subject: optical tweezers
phage phi 6
RNA packaging
dsRNA virus
RNA BACTERIOPHAGE PHI-6
PLUS-STRAND SYNTHESIS
3 GENOMIC SEGMENTS
DSRNA BACTERIOPHAGE-PHI-6
POLYMERASE COMPLEX
SYMMETRY MISMATCH
PURIFIED PROTEIN
INTERNAL FORCE
BINDING-SITES
MINUS-STRAND
1182 Biochemistry, cell and molecular biology
114 Physical sciences
Referentgranskad: Ja
Licens: cc_by_nc
Användningsbegränsning: openAccess
Parallelpublicerad version: publishedVersion


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