Single-molecule measurements of viral ssRNA packaging

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dc.contributor.author Hanhijarvi, Kalle J.
dc.contributor.author Ziedaite, Gabija
dc.contributor.author Bamford, Dennis H.
dc.contributor.author Haeggstrom, Edward
dc.contributor.author Poranen, Minna M.
dc.date.accessioned 2018-08-27T10:45:01Z
dc.date.available 2018-08-27T10:45:01Z
dc.date.issued 2017-01
dc.identifier.citation Hanhijarvi , K J , Ziedaite , G , Bamford , D H , Haeggstrom , E & Poranen , M M 2017 , ' Single-molecule measurements of viral ssRNA packaging ' , RNA , vol. 23 , no. 1 , pp. 119-129 . https://doi.org/10.1261/rna.057471.116
dc.identifier.other PURE: 79675251
dc.identifier.other PURE UUID: 0d2d28c6-8f8a-4710-a2d2-f0a34e39b56b
dc.identifier.other WOS: 000391687100012
dc.identifier.other Scopus: 85006971390
dc.identifier.other ORCID: /0000-0002-6438-8118/work/30261019
dc.identifier.other ORCID: /0000-0002-4775-3670/work/50290717
dc.identifier.uri http://hdl.handle.net/10138/239660
dc.description.abstract Genome packaging of double-stranded RNA (dsRNA) phages has been widely studied using biochemical and molecular biology methods. We adapted the existing in vitro packaging system of one such phage for single-molecule experimentation. To our knowledge, this is the first attempt to study the details of viral RNA packaging using optical tweezers. Pseudomonas phage phi 6 is a dsRNA virus with a tripartite genome. Positive-sense (+) single-stranded RNA (ssRNA) genome precursors are packaged into a preformed procapsid (PC), where negative strands are synthesized. We present single-molecule measurements of the viral ssRNA packaging by the phi 6 PC. Our data show that packaging proceeds intermittently in slow and fast phases, which likely reflects differences in the unfolding of the RNA secondary structures of the ssRNA being packaged. Although the mean packaging velocity was relatively low (0.07-0.54 nm/sec), packaging could reach 4.62 nm/sec during the fast packaging phase. en
dc.format.extent 11
dc.language.iso eng
dc.relation.ispartof RNA
dc.rights cc_by_nc
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject optical tweezers
dc.subject phage phi 6
dc.subject RNA packaging
dc.subject dsRNA virus
dc.subject RNA BACTERIOPHAGE PHI-6
dc.subject PLUS-STRAND SYNTHESIS
dc.subject 3 GENOMIC SEGMENTS
dc.subject DSRNA BACTERIOPHAGE-PHI-6
dc.subject POLYMERASE COMPLEX
dc.subject SYMMETRY MISMATCH
dc.subject PURIFIED PROTEIN
dc.subject INTERNAL FORCE
dc.subject BINDING-SITES
dc.subject MINUS-STRAND
dc.subject 1182 Biochemistry, cell and molecular biology
dc.subject 114 Physical sciences
dc.title Single-molecule measurements of viral ssRNA packaging en
dc.type Article
dc.contributor.organization Department of Physics
dc.contributor.organization Biosciences
dc.contributor.organization Institute of Biotechnology
dc.contributor.organization Structure of the Viral Universe
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1261/rna.057471.116
dc.relation.issn 1355-8382
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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