dc.contributor.author |
Hanhijarvi, Kalle J. |
|
dc.contributor.author |
Ziedaite, Gabija |
|
dc.contributor.author |
Bamford, Dennis H. |
|
dc.contributor.author |
Haeggstrom, Edward |
|
dc.contributor.author |
Poranen, Minna M. |
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dc.date.accessioned |
2018-08-27T10:45:01Z |
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dc.date.available |
2018-08-27T10:45:01Z |
|
dc.date.issued |
2017-01 |
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dc.identifier.citation |
Hanhijarvi , K J , Ziedaite , G , Bamford , D H , Haeggstrom , E & Poranen , M M 2017 , ' Single-molecule measurements of viral ssRNA packaging ' , RNA , vol. 23 , no. 1 , pp. 119-129 . https://doi.org/10.1261/rna.057471.116 |
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dc.identifier.other |
PURE: 79675251 |
|
dc.identifier.other |
PURE UUID: 0d2d28c6-8f8a-4710-a2d2-f0a34e39b56b |
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dc.identifier.other |
WOS: 000391687100012 |
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dc.identifier.other |
Scopus: 85006971390 |
|
dc.identifier.other |
ORCID: /0000-0002-6438-8118/work/30261019 |
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dc.identifier.other |
ORCID: /0000-0002-4775-3670/work/50290717 |
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dc.identifier.uri |
http://hdl.handle.net/10138/239660 |
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dc.description.abstract |
Genome packaging of double-stranded RNA (dsRNA) phages has been widely studied using biochemical and molecular biology methods. We adapted the existing in vitro packaging system of one such phage for single-molecule experimentation. To our knowledge, this is the first attempt to study the details of viral RNA packaging using optical tweezers. Pseudomonas phage phi 6 is a dsRNA virus with a tripartite genome. Positive-sense (+) single-stranded RNA (ssRNA) genome precursors are packaged into a preformed procapsid (PC), where negative strands are synthesized. We present single-molecule measurements of the viral ssRNA packaging by the phi 6 PC. Our data show that packaging proceeds intermittently in slow and fast phases, which likely reflects differences in the unfolding of the RNA secondary structures of the ssRNA being packaged. Although the mean packaging velocity was relatively low (0.07-0.54 nm/sec), packaging could reach 4.62 nm/sec during the fast packaging phase. |
en |
dc.format.extent |
11 |
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dc.language.iso |
eng |
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dc.relation.ispartof |
RNA |
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dc.rights |
cc_by_nc |
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dc.rights.uri |
info:eu-repo/semantics/openAccess |
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dc.subject |
optical tweezers |
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dc.subject |
phage phi 6 |
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dc.subject |
RNA packaging |
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dc.subject |
dsRNA virus |
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dc.subject |
RNA BACTERIOPHAGE PHI-6 |
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dc.subject |
PLUS-STRAND SYNTHESIS |
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dc.subject |
3 GENOMIC SEGMENTS |
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dc.subject |
DSRNA BACTERIOPHAGE-PHI-6 |
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dc.subject |
POLYMERASE COMPLEX |
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dc.subject |
SYMMETRY MISMATCH |
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dc.subject |
PURIFIED PROTEIN |
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dc.subject |
INTERNAL FORCE |
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dc.subject |
BINDING-SITES |
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dc.subject |
MINUS-STRAND |
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dc.subject |
1182 Biochemistry, cell and molecular biology |
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dc.subject |
114 Physical sciences |
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dc.title |
Single-molecule measurements of viral ssRNA packaging |
en |
dc.type |
Article |
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dc.contributor.organization |
Department of Physics |
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dc.contributor.organization |
Biosciences |
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dc.contributor.organization |
Institute of Biotechnology |
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dc.contributor.organization |
Structure of the Viral Universe |
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dc.description.reviewstatus |
Peer reviewed |
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dc.relation.doi |
https://doi.org/10.1261/rna.057471.116 |
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dc.relation.issn |
1355-8382 |
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dc.rights.accesslevel |
openAccess |
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dc.type.version |
publishedVersion |
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