Binding of EphrinA5 to RET receptor tyrosine kinase : An in vitro study

Show full item record



Permalink

http://hdl.handle.net/10138/239799

Citation

Liu , Y , Kaljunen , H , Pavic , A , Saarenpää , T , Himanen , J P , Nikolov , D B & Goldman , A 2018 , ' Binding of EphrinA5 to RET receptor tyrosine kinase : An in vitro study ' , PLoS One , vol. 13 , no. 6 , 0198291 . https://doi.org/10.1371/journal.pone.0198291

Title: Binding of EphrinA5 to RET receptor tyrosine kinase : An in vitro study
Author: Liu, Yixin; Kaljunen, Heidi; Pavic, Ana; Saarenpää, Tuulia; Himanen, Juha P.; Nikolov, Dimitar B.; Goldman, Adrian
Contributor: University of Helsinki, Molecular and Integrative Biosciences Research Programme
University of Helsinki, Molecular and Integrative Biosciences Research Programme
University of Helsinki, Molecular and Integrative Biosciences Research Programme
University of Helsinki, Molecular and Integrative Biosciences Research Programme
Date: 2018-06-11
Language: eng
Number of pages: 15
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/239799
Abstract: Eph/Ephrin signaling pathways are crucial in regulating a large variety of physiological processes during development, such as cell morphology, proliferation, migration and axonal guidance. EphrinA (efn-A) ligands, in particular, can be activated by EphA receptors at cellcell interfaces and have been proposed to cause reverse signaling via RET receptor tyrosine kinase. Such association has been reported to mediate spinal motor axon navigation, but conservation of the interactive signaling pathway and the molecular mechanism of the interaction are unclear. Here, we found Danio rerio efn-A5b bound to Mus musculus EphA4 with high affinity, revealing structurally and functionally conserved EphA/efn-A signaling. Interestingly, we observed no interaction between efn-A5b and RET from zebrafish, unlike earlier cell-based assays. Their lack of association indicates how complex efn-A signaling is and suggests that there may be other molecules involved in efn-A5-induced RET signaling.
Subject: GDNF FAMILY LIGANDS
BLUE-NATIVE PAGE
AXON GUIDANCE
EPH RECEPTORS
CELL-ADHESION
ACTIVATION
GDF15
SIGNALS
DISEASE
LIMB
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
file.pdf 3.572Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record