Cytoskeletal Stability in the Auditory Organ In Vivo : RhoA Is Dispensable for Wound Healing but Essential for Hair Cell Development

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Anttonen , T , Belevich , I , Laos , M , Herranen , A , Jokitalo , E , Brakebusch , C & Pirvola , U 2017 , ' Cytoskeletal Stability in the Auditory Organ In Vivo : RhoA Is Dispensable for Wound Healing but Essential for Hair Cell Development ' , eNeuro , vol. 4 , no. 5 , e0149-17.2017 . https://doi.org/10.1523/ENEURO.0149-17.2017

Title: Cytoskeletal Stability in the Auditory Organ In Vivo : RhoA Is Dispensable for Wound Healing but Essential for Hair Cell Development
Author: Anttonen, Tommi; Belevich, Ilya; Laos, Maarja; Herranen, Anni; Jokitalo, Eija; Brakebusch, Cord; Pirvola, Ulla
Contributor: University of Helsinki, Biosciences
University of Helsinki, Electron Microscopy
University of Helsinki, Biosciences
University of Helsinki, Biosciences
University of Helsinki, Institute of Biotechnology
University of Helsinki, Molecular and Integrative Biosciences Research Programme
Date: 2017
Language: eng
Number of pages: 17
Belongs to series: eNeuro
ISSN: 2373-2822
URI: http://hdl.handle.net/10138/240084
Abstract: Wound healing in the inner ear sensory epithelia is performed by the apical domains of supporting cells (SCs). Junctional F-actin belts of SCs are thin during development but become exceptionally thick during maturation. The functional significance of the thick belts is not fully understood. We have studied the role of F-actin belts during wound healing in the developing and adult cochlea of mice in vivo. We show that the thick belts serve as intracellular scaffolds that preserve the positions of surviving cells in the cochlear sensory epithelium. Junctions associated with the thick F-actin belts did not readily disassemble during wound healing. To compensate for this, basolateral membranes of SCs participated in the closure of surface breach. Because not only neighboring but also distant SCs contributed to wound healing by basolateral protrusions, this event appears to be triggered by contact-independent diffusible signals. In the search for regulators of wound healing, we inactivated RhoA in SCs, which, however, did not limit wound healing. RhoA inactivation in developing outer hair cells (OHCs) caused myosin II delocalization from the perijunctional domain and apical cell-surface enlargement. These abnormalities led to the extrusion of OHCs from the epithelium. These results demonstrate the importance of stability of the apical domain, both in wound repair by SCs and in development of OHCs, and that only this latter function is regulated by RhoA. Because the correct cytoarchitecture of the cochlear sensory epithelium is required for normal hearing, the stability of cell apices should be maintained in regenerative and protective interventions.
Subject: Auditory
cytoskeleton
development
inner ear
RhoA
wound healing
INNER-EAR
APICAL CONSTRICTION
SUPPORTING CELLS
SINGLE-CELL
POLARITY
MOUSE
JUNCTIONS
COCHLEA
MORPHOGENESIS
ORGANIZATION
3112 Neurosciences
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