Membrane-containing virus particles exhibit the mechanics of a composite material for genome protection

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Azinas , S , Bano , F , Torca , I , Bamford , D H , Schwartz , G A , Esnaola , J , Oksanen , H M , Richter , R P & Abrescia , N G 2018 , ' Membrane-containing virus particles exhibit the mechanics of a composite material for genome protection ' , Nanoscale , vol. 10 , no. 16 , pp. 7769-7779 . https://doi.org/10.1039/c8nr00196k

Title: Membrane-containing virus particles exhibit the mechanics of a composite material for genome protection
Author: Azinas, S.; Bano, F.; Torca, I.; Bamford, D. H.; Schwartz, G. A.; Esnaola, J.; Oksanen, H. M.; Richter, R. P.; Abrescia, N. G.
Other contributor: University of Helsinki, Biosciences
University of Helsinki, Molecular and Integrative Biosciences Research Programme




Date: 2018-04-28
Language: eng
Number of pages: 11
Belongs to series: Nanoscale
ISSN: 2040-3364
DOI: https://doi.org/10.1039/c8nr00196k
URI: http://hdl.handle.net/10138/240369
Abstract: The protection of the viral genome during extracellular transport is an absolute requirement for virus survival and replication. In addition to the almost universal proteinaceous capsids, certain viruses add a membrane layer that encloses their double-stranded (ds) DNA genome within the protein shell. Using the membrane-containing enterobacterial virus PRD1 as a prototype, and a combination of nanoindentation assays by atomic force microscopy and finite element modelling, we show that PRD1 provides a greater stability against mechanical stress than that achieved by the majority of dsDNA icosahedral viruses that lack a membrane. We propose that the combination of a stiff and brittle proteinaceous shell coupled with a soft and compliant membrane vesicle yields a tough composite nanomaterial well-suited to protect the viral DNA during extracellular transport.
Subject: ATOMIC-FORCE MICROSCOPY
BACTERIOPHAGE PRD1
HUMAN ADENOVIRUS
WILD-TYPE
PROTEIN
DNA
CAPSIDS
PHAGE
REINFORCEMENT
QUANTITATION
1182 Biochemistry, cell and molecular biology
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