Size-exclusion chromatography of xylan derivatives-the critical evaluation of macromolecular data

Show simple item record

dc.contributor.author Pitkänen, Leena Maria
dc.contributor.author Aseyev, Vladimir
dc.contributor.author Laine, Christiane
dc.contributor.author Tuomainen, Päivi Marja Sinikka
dc.contributor.author Tenkanen, Tiina Maija
dc.date.accessioned 2018-09-05T13:06:01Z
dc.date.available 2018-09-05T13:06:01Z
dc.date.issued 2017
dc.identifier.citation Pitkänen , L M , Aseyev , V , Laine , C , Tuomainen , P M S & Tenkanen , T M 2017 , ' Size-exclusion chromatography of xylan derivatives-the critical evaluation of macromolecular data ' , Analytical and Bioanalytical Chemistry , vol. 409 , no. 20 , pp. 4811–4817 . https://doi.org/10.1007/s00216-017-0424-5
dc.identifier.other PURE: 85693683
dc.identifier.other PURE UUID: 180fec90-dc23-43bb-9bd1-0e290f47cd97
dc.identifier.other Scopus: 85020115239
dc.identifier.other WOS: 000406146800013
dc.identifier.other ORCID: /0000-0002-3739-8089/work/39201524
dc.identifier.other ORCID: /0000-0003-2883-2717/work/39203868
dc.identifier.uri http://hdl.handle.net/10138/241065
dc.description.abstract Hydroxypropyl xylans with varying degrees of substitution were characterized by size-exclusion chromatography. Molar masses of the samples were determined using two approaches: by conventional calibration with molar mass standards and by a multi-detection method that utilizes the combination of static light scattering, viscometry, and differential refractive index detection. The molar mass results obtained by the multi-detection method were accurate, but required the determination of separate refractive index increments for each structurally different sample. The column calibration approach with standard pullulan samples gave biased results due to the differences in hydrodynamic volumes between pullulans and hydroxypropyl xylans with similar molar masses. The degree of hydroxypropylation affected the chain conformation and compactness of the polymer chains. Mark-Houwink parameters and persistence length values suggested that the hydroxypropyl substituents reduced the flexibility of the xylan chain and made the polymer chain more extended. en
dc.format.extent 7
dc.language.iso eng
dc.relation.ispartof Analytical and Bioanalytical Chemistry
dc.rights unspecified
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 116 Chemical sciences
dc.title Size-exclusion chromatography of xylan derivatives-the critical evaluation of macromolecular data en
dc.type Article
dc.contributor.organization Department of Food and Nutrition
dc.contributor.organization Department of Chemistry
dc.contributor.organization Vladimir Aseyev / Principal Investigator
dc.contributor.organization Laboratory of Polymer Chemistry (-2016)
dc.contributor.organization Carbohydrate Chemistry and Enzymology
dc.contributor.organization Polymers
dc.contributor.organization Food Sciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1007/s00216-017-0424-5
dc.relation.issn 1618-2642
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
HPXyl_Pitk_nen_ABC_2017.pdf 868.3Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record