Sialic Acid-Like Sugars in Archaea : Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp. PV6

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Zaretsky , M , Roine , E & Eichler , J 2018 , ' Sialic Acid-Like Sugars in Archaea : Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp. PV6 ' , Frontiers in Microbiology , vol. 9 , 2133 . https://doi.org/10.3389/fmicb.2018.02133

Title: Sialic Acid-Like Sugars in Archaea : Legionaminic Acid Biosynthesis in the Halophile Halorubrum sp. PV6
Author: Zaretsky, Marianna; Roine, Elina; Eichler, Jerry
Contributor: University of Helsinki, Molecular and Integrative Biosciences Research Programme
Date: 2018-09-07
Language: eng
Number of pages: 13
Belongs to series: Frontiers in Microbiology
ISSN: 1664-302X
URI: http://hdl.handle.net/10138/244924
Abstract: N-glycosylation is a post-translational modification that occurs in all three domains. In Archaea, however, N-linked glycans present a degree of compositional diversity not observed in either Eukarya or Bacteria. As such, it is surprising that nonulosonic acids (NulOs), nine-carbon sugars that include sialic acids, pseudaminic acids, and legionaminic acids, are routinely detected as components of protein-linked glycans in Eukarya and Bacteria but not in Archaea. In the following, we report that the N-linked glycan attached to the S-layer glycoprotein of the haloarchaea Halorubrum sp. PV6 includes an N-formylated legionaminic acid. Analysis of the Halorubrum sp. PV6 genome led to the identification of sequences predicted to comprise the legionaminic acid biosynthesis pathway. The transcription of pathway genes was confirmed, as was the co-transcription of several of these genes. In addition, the activities of LegI, which catalyzes the condensation of 2,4-di-N-acetyl-6-deoxymannose and phosphoenolpyruvate to generate legionaminic acid, and LegF, which catalyzes the addition of cytidine monophosphate (CMP) to legionaminic acid, both heterologously expressed in Haloferax volcanii, were demonstrated. Further genome analysis predicts that the genes encoding enzymes of the legionaminic acid biosynthetic pathway are clustered together with sequences seemingly encoding components of the N-glycosylation pathway in this organism. In defining the first example of a legionaminic acid biosynthesis pathway in Archaea, the findings reported here expand our insight into archaeal N-glycosylation, an almost universal post-translational modification in this domain of life.
Subject: archaea
halophile
Halorubrum
legionaminic acid
N-formylation
N-glycosylation
S-layer glycoprotein
ESCHERICHIA-COLI K1
LINKED GLYCOSYLATION PATHWAY
HALOFERAX-VOLCANII
N-GLYCOSYLATION
PROTEIN GLYCOSYLATION
LAYER GLYCOPROTEIN
METHANOBREVIBACTER-SMITHII
LEGIONELLA-PNEUMOPHILA
CAMPYLOBACTER-JEJUNI
SYNTHETASE
1183 Plant biology, microbiology, virology
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