Compensating for cross-reactions using avidity and computation in a suspension multiplex immunoassay for serotyping of Zika versus other flavivirus infections

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Ronnberg , B , Gustafsson , A , Vapalahti , O , Emmerich , P , Lundkvist , A , Schmidt-Chanasit , J & Blomberg , J 2017 , ' Compensating for cross-reactions using avidity and computation in a suspension multiplex immunoassay for serotyping of Zika versus other flavivirus infections ' , Medical Microbiology and Immunology , vol. 206 , no. 5 , pp. 383-401 . https://doi.org/10.1007/s00430-017-0517-y

Title: Compensating for cross-reactions using avidity and computation in a suspension multiplex immunoassay for serotyping of Zika versus other flavivirus infections
Author: Ronnberg, Bengt; Gustafsson, Ake; Vapalahti, Olli; Emmerich, Petra; Lundkvist, Ake; Schmidt-Chanasit, Jonas; Blomberg, Jonas
Contributor: University of Helsinki, Medicum
Date: 2017-10
Language: eng
Number of pages: 19
Belongs to series: Medical Microbiology and Immunology
ISSN: 0300-8584
URI: http://hdl.handle.net/10138/245624
Abstract: The recent spread of Zika virus (ZIKV) in the Americas and Asia necessitates an increased preparedness for improved maternal and perinatal health and blood safety. However, serological cross-reactions, especially to Dengue virus (DENV), complicate ZIKV antibody serodiagnosis. A novel "pan-Flavi" suspension multiplex immunoassay (PFSMIA) using 25 antigens, whole virus (WV), non-structural protein 1 (NS1), and envelope (E) proteins, from 7 zoonotic flaviviruses for specific detection of ZIKV and DENV IgM and IgG was developed. Patterns of antibody cross-reactivity, avidity, and kinetics were established in 104 sera from returning travelers with known ZIKV and DENV infections. PFSMIA gave IgM- and IgG-sensitivities for both viruses of 96-100%, compared to an immunofluorescence assay. Main IgM cross-reactions were to NS1, for IgG to the E and WV antigens. Infecting virus yielded reactivity to several antigens of the homologous virus, while cross-reactions tended to occur only to a single antigen from heterologous virus(es). A specificity-enhancing computer procedure took into account antibody isotype, number of antibody-reactive antigens per virus, avidity, average degree of cross-reactivity to heterologous flavivirus antigens, and reactivity changes in serial sera. It classified all 50 cases correctly. Applied to sera from 200 pregnant women and 173 blood donors from Sweden, one blood donor was found ZIKV NS1 IgM positive, and another as ZIKV NS1 IgG positive. These samples did not react with other ZIKV antigens and were thereby judged as false-positives. PFSMIA provided sensitive and specific ZIKV and DENV serology, warranting high-throughput serological surveillance and a minimized need for laborious and expensive virus neutralization assays.
Subject: Zika virus
Dengue virus
Flavivirus
Suspension multiplex immunoassay
Serological crossreaction
Pathogen surveillance
WEST NILE VIRUS
IMMUNOGLOBULIN-G
DENGUE VIRUS
REACTIVITY
ANTIBODIES
CHALLENGE
SEROLOGY
THREAT
ARRAY
1183 Plant biology, microbiology, virology
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