Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains

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Razzauti Sanfeliu , M , Plyusnina , A , Sironen , T , Henttonen , H & Pljusnin , A 2009 , ' Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains ' , Journal of General Virology , vol. 90 , no. 8 , pp. 1923-1931 . https://doi.org/10.1099/vir.0.011304-0

Title: Analysis of Puumala hantavirus in a bank vole population in northern Finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains
Author: Razzauti Sanfeliu, Maria; Plyusnina, Angelina; Sironen, Tarja; Henttonen, Heikki; Pljusnin, Aleksandr
Contributor: University of Helsinki, Department of Virology
University of Helsinki, Department of Virology
University of Helsinki, Department of Virology
University of Helsinki, Department of Virology
Date: 2009
Language: eng
Number of pages: 9
Belongs to series: Journal of General Virology
ISSN: 0022-1317
URI: http://hdl.handle.net/10138/24590
Abstract: "In this study, for the first time, two distinct genetic lineages of Puumala virus (PUUV) were found within a small sampling area and within a single host genetic lineage (Ural mtDNA) at Pallasjarvi, northern Finland. Lung tissue samples of 171 bank voles (Myodes glareolus) trapped in September 1998 were screened for the presence of PUUV nucleocapsid antigen and 25 were found to be positive. Partial sequences of the PUUV small (S), medium (M) and large (L) genome segments were recovered from these samples using RT-PCR. Phylogenetic analysis revealed two genetic groups of PUUV sequences that belonged to the Finnish and north Scandinavian lineages. This presented a unique opportunity to study inter-lineage reassortment in PUUV; indeed, 32% of the studied bank voles appeared to carry reassortant virus genomes. Thus, the frequency of inter-lineage reassortment in PUUV was comparable to that of intra-lineage reassortment observed previously (Razzauti, M., Plyusnina, A., Henttonen, H. & Plyusnin, A. (2008). J Gen Virol 89, 1649-1660). Of six possible reassortant S/M/L combinations, only two were found at Pallasjarvi and, notably, in all reassortants, both S and L segments originated from the same genetic lineage, suggesting a non-random pattern for the reassortment. These findings are discussed in connection to PUUV evolution in Fermoscandia."
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