Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer

Show full item record



Permalink

http://hdl.handle.net/10138/246368

Citation

Carnielli , C M , Soares Macedo , C C , De Rossi , T , Granato , D C , Rivera , C , Domingues , R R , Pauletti , B A , Yokoo , S , Heberle , H , Busso-Lopes , A F , Cervigne , N K , Sawazaki-Calone , I , Meirelles , G V , Marchi , F A , Telles , G P , Minghim , R , Prado Ribeiro , A C , Brandao , T B , Castro , G D , Alejandro Gonzalez-Arriagada , W , Gomes , A , Penteado , F , Santos-Silva , A R , Lopes , M A , Rodrigues , P C , Sundquist , E , Salo , T , da Silva , S D , Alaoui-Jamali , M A , Graner , E , Fox , J W , Della Coletta , R & Paes Leme , A F 2018 , ' Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer ' , Nature Communications , vol. 9 , 3598 . https://doi.org/10.1038/s41467-018-05696-2

Title: Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer
Author: Carnielli, Carolina Moretto; Soares Macedo, Carolina Carneiro; De Rossi, Tatiane; Granato, Daniela Campos; Rivera, Cesar; Domingues, Romenia Ramos; Pauletti, Bianca Alves; Yokoo, Sami; Heberle, Henry; Busso-Lopes, Ariane Fidelis; Cervigne, Nilva Karla; Sawazaki-Calone, Iris; Meirelles, Gabriela Vaz; Marchi, Fabio Albuquerque; Telles, Guilherme Pimentel; Minghim, Rosane; Prado Ribeiro, Ana Carolina; Brandao, Thais Bianca; Castro, Gilberto de; Alejandro Gonzalez-Arriagada, Wilfredo; Gomes, Alexandre; Penteado, Fabio; Santos-Silva, Alan Roger; Lopes, Marcio Ajudarte; Rodrigues, Priscila Campioni; Sundquist, Elias; Salo, Tuula; da Silva, Sabrina Daniela; Alaoui-Jamali, Moulay A.; Graner, Edgard; Fox, Jay W.; Della Coletta, Ricardo; Paes Leme, Adriana Franco
Contributor: University of Helsinki, Clinicum
Date: 2018-09-05
Language: eng
Number of pages: 17
Belongs to series: Nature Communications
ISSN: 2041-1723
URI: http://hdl.handle.net/10138/246368
Abstract: Different regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor-node-metastasis prognosis classification. Therefore, defining biological signatures that allow assessing the prognostic outcomes for OSCC patients would be of great clinical significance. Using histopathology-guided discovery proteomics, we analyze neoplastic islands and stroma from the invasive tumor front (ITF) and inner tumor to identify differentially expressed proteins. Potential signature proteins are prioritized and further investigated by immunohistochemistry (IHC) and targeted proteomics. IHC indicates low expression of cystatin-B in neoplastic islands from the ITF as an independent marker for local recurrence. Targeted proteomics analysis of the prioritized proteins in saliva, combined with machine-learning methods, highlights a peptide-based signature as the most powerful predictor to distinguish patients with and without lymph node metastasis. In summary, we identify a robust signature, which may enhance prognostic decisions in OSCC and better guide treatment to reduce tumor recurrence or lymph node metastasis.
Subject: SQUAMOUS-CELL CARCINOMA
EPITHELIAL-MESENCHYMAL TRANSITION
TUMOR MICROENVIRONMENT
QUANTITATIVE PROTEOMICS
INVASIVE FRONT
TONGUE CANCER
COLLAGEN-VI
METASTASIS
PROGRESSION
OVEREXPRESSION
3122 Cancers
313 Dentistry
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
s41467_018_05696_2.pdf 3.059Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record