Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer

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Carnielli , C M , Soares Macedo , C C , De Rossi , T , Granato , D C , Rivera , C , Domingues , R R , Pauletti , B A , Yokoo , S , Heberle , H , Busso-Lopes , A F , Cervigne , N K , Sawazaki-Calone , I , Meirelles , G V , Marchi , F A , Telles , G P , Minghim , R , Prado Ribeiro , A C , Brandao , T B , Castro , G D , Alejandro Gonzalez-Arriagada , W , Gomes , A , Penteado , F , Santos-Silva , A R , Lopes , M A , Rodrigues , P C , Sundquist , E , Salo , T , da Silva , S D , Alaoui-Jamali , M A , Graner , E , Fox , J W , Della Coletta , R & Paes Leme , A F 2018 , ' Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer ' , Nature Communications , vol. 9 , 3598 .

Title: Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer
Author: Carnielli, Carolina Moretto; Soares Macedo, Carolina Carneiro; De Rossi, Tatiane; Granato, Daniela Campos; Rivera, Cesar; Domingues, Romenia Ramos; Pauletti, Bianca Alves; Yokoo, Sami; Heberle, Henry; Busso-Lopes, Ariane Fidelis; Cervigne, Nilva Karla; Sawazaki-Calone, Iris; Meirelles, Gabriela Vaz; Marchi, Fabio Albuquerque; Telles, Guilherme Pimentel; Minghim, Rosane; Prado Ribeiro, Ana Carolina; Brandao, Thais Bianca; Castro, Gilberto de; Alejandro Gonzalez-Arriagada, Wilfredo; Gomes, Alexandre; Penteado, Fabio; Santos-Silva, Alan Roger; Lopes, Marcio Ajudarte; Rodrigues, Priscila Campioni; Sundquist, Elias; Salo, Tuula; da Silva, Sabrina Daniela; Alaoui-Jamali, Moulay A.; Graner, Edgard; Fox, Jay W.; Della Coletta, Ricardo; Paes Leme, Adriana Franco
Contributor organization: Clinicum
Department of Oral and Maxillofacial Diseases
University of Helsinki
Date: 2018-09-05
Language: eng
Number of pages: 17
Belongs to series: Nature Communications
ISSN: 2041-1723
Abstract: Different regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor-node-metastasis prognosis classification. Therefore, defining biological signatures that allow assessing the prognostic outcomes for OSCC patients would be of great clinical significance. Using histopathology-guided discovery proteomics, we analyze neoplastic islands and stroma from the invasive tumor front (ITF) and inner tumor to identify differentially expressed proteins. Potential signature proteins are prioritized and further investigated by immunohistochemistry (IHC) and targeted proteomics. IHC indicates low expression of cystatin-B in neoplastic islands from the ITF as an independent marker for local recurrence. Targeted proteomics analysis of the prioritized proteins in saliva, combined with machine-learning methods, highlights a peptide-based signature as the most powerful predictor to distinguish patients with and without lymph node metastasis. In summary, we identify a robust signature, which may enhance prognostic decisions in OSCC and better guide treatment to reduce tumor recurrence or lymph node metastasis.
3122 Cancers
313 Dentistry
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion

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