Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome

Show full item record



Permalink

http://hdl.handle.net/10138/246372

Citation

Woitowich , N C , Halavaty , A S , Waltz , P , Kupitz , C , Valera , J , Tracy , G , Gallagher , K D , Claesson , E , Nakane , T , Pandey , S , Nelson , G , Tanaka , R , Nango , E , Mizohata , E , Owada , S , Tono , K , Joti , Y , Nugent , A C , Patel , H , Mapara , A , Hopkins , J , Duong , P , Bizhga , D , Kovaleva , S E , St Peter , R , Hernandez , C N , Ozarowski , W B , Roy-Chowdhuri , S , Yang , J-H , Edlund , P , Takala , H , Ihalainen , J , Brayshaw , J , Norwood , T , Poudyal , I , Fromme , P , Spence , J C H , Moffat , K , Westenhoff , S , Schmidt , M & Stojkovic , E A 2018 , ' Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome ' , IUCrJ. , vol. 5 , no. 5 , pp. 619-634 . https://doi.org/10.1107/S2052252518010631

Title: Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome
Author: Woitowich, Nicole C.; Halavaty, Andrei S.; Waltz, Patricia; Kupitz, Christopher; Valera, Joseph; Tracy, Gregory; Gallagher, Kevin D.; Claesson, Elin; Nakane, Takanori; Pandey, Suraj; Nelson, Garrett; Tanaka, Rie; Nango, Eriko; Mizohata, Eiichi; Owada, Shigeki; Tono, Kensure; Joti, Yasumasa; Nugent, Angela C.; Patel, Hardik; Mapara, Ayesha; Hopkins, James; Duong, Phu; Bizhga, Dorina; Kovaleva, Svetlana E.; St Peter, Rachael; Hernandez, Cynthia N.; Ozarowski, Wesley B.; Roy-Chowdhuri, Shatabdi; Yang, Jay-How; Edlund, Petra; Takala, Heikki; Ihalainen, Janne; Brayshaw, Jennifer; Norwood, Tyler; Poudyal, Ishwor; Fromme, Petra; Spence, John C. H.; Moffat, Keith; Westenhoff, Sebastian; Schmidt, Marius; Stojkovic, Emina A.
Contributor: University of Helsinki, Medicum
Date: 2018-09
Language: eng
Number of pages: 16
Belongs to series: IUCrJ.
ISSN: 2052-2525
URI: http://hdl.handle.net/10138/246372
Abstract: Phytochromes are red-light photoreceptors that were first characterized in plants, with homologs in photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs interconvert between two states denoted Pr and Pfr with distinct absorption spectra in the red and far-red. They have recently been engineered as enzymatic photoswitches for fluorescent-marker applications in non-invasive tissue imaging of mammals. This article presents cryo- and room-temperature crystal structures of the unusual phytochrome from the non-photosynthetic myxobacterium Stigmatella aurantiaca (SaBphP1) and reveals its role in the fruitingbody formation of this photomorphogenic bacterium. SaBphP1 lacks a conserved histidine (His) in the chromophore-binding domain that stabilizes the Pr state in the classical BphPs. Instead it contains a threonine (Thr), a feature that is restricted to several myxobacterial phytochromes and is not evolutionarily understood. SaBphP1 structures of the chromophore binding domain (CBD) and the complete photosensory core module (PCM) in wild-type and Thr-to-His mutant forms reveal details of the molecular mechanism of the Pr/Pfr transition associated with the physiological response of this myxobacterium to red light. Specifically, key structural differences in the CBD and PCM between the wild-type and the Thr-to-His mutant involve essential chromophore contacts with proximal amino acids, and point to how the photosignal is transduced through the rest of the protein, impacting the essential enzymatic activity in the photomorphogenic response of this myxobacterium.
Subject: phytochromes
photoreceptors
photosynthetic bacteria
myxobacteria
absorption spectra
PHOTOACTIVE YELLOW PROTEIN
CHROMOPHORE-BINDING DOMAIN
FRUITING BODY FORMATION
STIGMATELLA-AURANTIACA
UNUSUAL BACTERIOPHYTOCHROME
MOLECULAR REPLACEMENT
SIGNALING MECHANISM
PHOTOCONVERSION
CRYSTALLOGRAPHY
MODULE
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
jt5025.pdf 2.066Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record