Actinobacteria associated with Chinaberry tree are diverse and show antimicrobial activity

Show simple item record

dc.contributor University of Helsinki, Ecosystems and Environment Research Programme en
dc.contributor.author Zhao, Ke
dc.contributor.author Li, Jing
dc.contributor.author Shen, Meiling
dc.contributor.author Chen, Qiang
dc.contributor.author Liu, Maoke
dc.contributor.author Ao, Xiaolin
dc.contributor.author Liao, Decong
dc.contributor.author Gu, Yunfu
dc.contributor.author Xu, Kaiwei
dc.contributor.author Ma, Menggen
dc.contributor.author Yu, Xiumei
dc.contributor.author Xiang, Quanju
dc.contributor.author Chen, Ji
dc.contributor.author Zhang, Xiaoping
dc.contributor.author Penttinen, Petri
dc.date.accessioned 2018-10-03T08:27:01Z
dc.date.available 2018-10-03T08:27:01Z
dc.date.issued 2018-07-23
dc.identifier.citation Zhao , K , Li , J , Shen , M , Chen , Q , Liu , M , Ao , X , Liao , D , Gu , Y , Xu , K , Ma , M , Yu , X , Xiang , Q , Chen , J , Zhang , X & Penttinen , P 2018 , ' Actinobacteria associated with Chinaberry tree are diverse and show antimicrobial activity ' , Scientific Reports , vol. 8 , 11103 . https://doi.org/10.1038/s41598-018-29442-2 en
dc.identifier.issn 2045-2322
dc.identifier.other PURE: 116331416
dc.identifier.other PURE UUID: 57de394b-5bce-4150-9af7-3679e7dc3564
dc.identifier.other WOS: 000439421600071
dc.identifier.other Scopus: 85050600305
dc.identifier.uri http://hdl.handle.net/10138/246609
dc.description.abstract Many actinobacteria produce secondary metabolites that include antimicrobial compounds. Since most of the actinobacteria cannot be cultivated, their antimicrobial potential awaits to be revealed. We hypothesized that the actinobacterial endophyte communities inside Melia toosendan (Chinaberry) tree are diverse, include strains with antimicrobial activity, and that antimicrobial activity can be detected using a cultivation independent approach and co-occurrence analysis. We isolated and identified actinobacteria from Chinaberry, tested their antimicrobial activities, and characterized the communities using amplicon sequencing and denaturing gradient gel electrophoresis as cultivation independent methods. Most of the isolates were identified as Streptomyces spp., whereas based on amplicon sequencing the most abundant OTU was assigned to Rhodococcus, and Tomitella was the most diverse genus. Out of the 135 isolates, 113 inhibited the growth of at least one indicator organism. Six out of the 7577 operational taxonomic units (OTUs) matched 46 cultivated isolates. Only three OTUs, Streptomyces OTU4, OTU11, and OTU26, and their corresponding isolate groups were available for comparing co-occurrences and antimicrobial activity. Streptomyces OTU4 correlated negatively with a high number of OTUs, and the isolates corresponding to Streptomyces OTU4 had high antimicrobial activity. However, for the other two OTUs and their corresponding isolate groups there was no clear relation between the numbers of negative correlations and antimicrobial activity. Thus, the applicability of co-occurrence analysis in detecting antimicrobially active actinobacteria could not be proven. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights en
dc.subject MEDICINAL-PLANTS en
dc.subject ENDOPHYTIC ACTINOBACTERIA en
dc.subject ANTIBIOTIC-RESISTANCE en
dc.subject SP NOV. en
dc.subject RNA en
dc.subject XISHUANGBANNA en
dc.subject STREPTOMYCES en
dc.subject COMMUNITIES en
dc.subject BACTERIA en
dc.subject PANXI en
dc.subject 1183 Plant biology, microbiology, virology en
dc.title Actinobacteria associated with Chinaberry tree are diverse and show antimicrobial activity en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1038/s41598-018-29442-2
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
dc.contributor.pbl

Files in this item

Total number of downloads: Loading...

Files Size Format View
s41598_018_29442_2.pdf 1.867Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record