Metabolism and phospholipid assembly of polyunsaturated fatty acids in human bone marrow mesenchymal stromal cells

Show full item record



Permalink

http://hdl.handle.net/10138/247816

Citation

Tigistu-Sahle , F , Lampinen , M , Kilpinen , L , Holopainen , M , Lehenkari , P , Laitinen , S & Käkelä , R 2017 , ' Metabolism and phospholipid assembly of polyunsaturated fatty acids in human bone marrow mesenchymal stromal cells ' , Journal of Lipid Research , vol. 58 , no. 1 , pp. 92-110 . https://doi.org/10.1194/jlr.M070680

Title: Metabolism and phospholipid assembly of polyunsaturated fatty acids in human bone marrow mesenchymal stromal cells
Author: Tigistu-Sahle, Feven; Lampinen, Milla; Kilpinen, Lotta; Holopainen, Minna; Lehenkari, Petri; Laitinen, Saara; Käkelä, Reijo
Other contributor: University of Helsinki, Biosciences
University of Helsinki, Biosciences
University of Helsinki, Biosciences
University of Helsinki, Finnish Red Cross Blood Service
University of Helsinki, Functional Lipidomics Group


Date: 2017-01
Language: eng
Number of pages: 19
Belongs to series: Journal of Lipid Research
ISSN: 0022-2275
DOI: https://doi.org/10.1194/jlr.M070680
URI: http://hdl.handle.net/10138/247816
Abstract: High arachidonic acid (20:4n-6) and low n-3 PUFA levels impair the capacity of cultured human bone marrow mesenchymal stromal cells (hBMSCs) to modulate immune functions. The capacity of the hBMSCs to modify PUFA structures was found to be limited. Therefore, different PUFA supplements given to the cells resulted in very different glycerophospholipid (GPL) species profiles and substrate availability for phospholipases, which have preferences for polar head group and acyl chains when liberating PUFA precursors for production of lipid mediators. When supplemented with 20:4n-6, the cells increased prostaglandin E2 secretion. However, they elongated 20:4n-6 to the less active precursor, 22:4n-6, and also incorporated it into triacylglycerols, which may have limited the proinflammatory signaling. The n-3 PUFA precursor, 18:3n-3, had little potency to reduce the GPL 20:4n-6 content, while the eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acid supplements efficiently displaced the 20:4n-6 acyls, and created diverse GPL species substrate pools allowing attenuation of inflammatory signaling.(Jlr) The results emphasize the importance of choosing appropriate PUFA supplements for in vitro hBMSC expansion and suggests that for optimal function they require an exogenous fatty acid source providing 20:5n-3 and 22:6n-3 sufficiently, but 20:4n-6 moderately, which calls for specifically designed optimal PUFA supplements for the cultures.
Subject: arachidonic acid
docosahexaenoic acid
eicosapentaenoic acid
glycerophospholipid
immunomodulation
lipid signaling
mass spectrometry
mesenchymal stromal/stem cell
prostaglandin E2
TANDEM MASS-SPECTROMETRY
HEMATOPOIETIC STEM-CELLS
PROTEIN-KINASE-C
ARACHIDONIC-ACID
PROGENITOR CELLS
LIPID RAFTS
ELECTROSPRAY-IONIZATION
INFLAMMATORY CELLS
NITRIC-OXIDE
RAT-LIVER
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
J._Lipid_Res._2017_Tigistu_Sahle_92_110.pdf 2.590Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record