Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing

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Chen , W , Duša , F , Witos , J M , Ruokonen , S-K & Wiedmer , S K 2018 , ' Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing ' , Scientific Reports , vol. 8 , 14815 . https://doi.org/10.1038/s41598-018-33107-5

Title: Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing
Author: Chen, Wen; Duša, Filip; Witos, Joanna Magdalena; Ruokonen, Suvi-Katriina; Wiedmer, Susanne Kristina
Contributor: University of Helsinki, Department of Chemistry
University of Helsinki, Aalto University
University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
Date: 2018-10-04
Language: eng
Number of pages: 11
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/250557
Abstract: Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (Tm) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the Tm of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the Tm of the lipid. The results show that, when the system temperature approaches the Tm, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 °C, 41.0 °C, and 55.5 °C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical Tm values, i.e., 24 °C, 41.4 °C, and 55 °C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the Tm of phospholipids.
Subject: 116 Chemical sciences
DIFFERENTIAL SCANNING CALORIMETRY
QUARTZ-CRYSTAL MICROBALANCE
SUPPORTED LIPID-BILAYERS
DRUG-DELIVERY SYSTEMS
IONIC LIQUIDS
NANOMATERIALS SCIENCE
BIOLOGICAL-MEMBRANES
MODEL MEMBRANES
VESICLES
LIPOSOME
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