How to minimize dye-induced perturbations while studying biomembrane structure and dynamics : PEG linkers as a rational alternative

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Mobarak , E , Javanainen , M , Kulig , W , Honigmann , A , Sezgin , E , Aho , N , Eggeling , C , Rog , T & Vattulainen , I 2018 , ' How to minimize dye-induced perturbations while studying biomembrane structure and dynamics : PEG linkers as a rational alternative ' , Biochimica et Biophysica Acta. Biomembranes , vol. 1860 , no. 11 , pp. 2436-2445 . https://doi.org/10.1016/j.bbamem.2018.07.003

Title: How to minimize dye-induced perturbations while studying biomembrane structure and dynamics : PEG linkers as a rational alternative
Author: Mobarak, Edouard; Javanainen, Matti; Kulig, Waldemar; Honigmann, Alf; Sezgin, Erdinc; Aho, Noora; Eggeling, Christian; Rog, Tomasz; Vattulainen, Ilpo
Contributor: University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
Date: 2018-11
Language: eng
Number of pages: 10
Belongs to series: Biochimica et Biophysica Acta. Biomembranes
ISSN: 0005-2736
URI: http://hdl.handle.net/10138/254905
Abstract: Organic dye-tagged lipid analogs are essential for many fluorescence-based investigations of complex membrane structures, especially when using advanced microscopy approaches. However, lipid analogs may interfere with membrane structure and dynamics, and it is not obvious that the properties of lipid analogs would match those of non-labeled host lipids. In this work, we bridged atomistic simulations with super-resolution imaging experiments and biomimetic membranes to assess the performance of commonly used sphingomyelin-based lipid analogs. The objective was to compare, on equal footing, the relative strengths and weaknesses of acyl chain labeling, headgroup labeling, and labeling based on poly-ethyl-glycol (PEG) linkers in determining biomembrane properties. We observed that the most appropriate strategy to minimize dye-induced membrane perturbations and to allow consideration of Brownian-like diffusion in liquid-ordered membrane environments is to decouple the dye from a membrane by a PEG linker attached to a lipid headgroup. Yet, while the use of PEG linkers may sound a rational and even an obvious approach to explore membrane dynamics, the results also suggest that the dyes exploiting PEG linkers interfere with molecular interactions and their dynamics. Overall, the results highlight the great care needed when using fluorescent lipid analogs, in particular accurate controls.
Subject: Fluorescent probe
PEG linker
Lipid membrane
Atomistic simulation
Molecular dynamics simulation
Super-resolution microscopy
114 Physical sciences
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