How to minimize dye-induced perturbations while studying biomembrane structure and dynamics : PEG linkers as a rational alternative

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dc.contributor University of Helsinki, Department of Physics en
dc.contributor University of Helsinki, Department of Physics en
dc.contributor University of Helsinki, Department of Physics en
dc.contributor University of Helsinki, Department of Physics en
dc.contributor University of Helsinki, Department of Physics en
dc.contributor University of Helsinki, Department of Physics en
dc.contributor.author Mobarak, Edouard
dc.contributor.author Javanainen, Matti
dc.contributor.author Kulig, Waldemar
dc.contributor.author Honigmann, Alf
dc.contributor.author Sezgin, Erdinc
dc.contributor.author Aho, Noora
dc.contributor.author Eggeling, Christian
dc.contributor.author Rog, Tomasz
dc.contributor.author Vattulainen, Ilpo
dc.date.accessioned 2018-10-29T09:45:01Z
dc.date.available 2018-10-29T09:45:01Z
dc.date.issued 2018-11
dc.identifier.citation Mobarak , E , Javanainen , M , Kulig , W , Honigmann , A , Sezgin , E , Aho , N , Eggeling , C , Rog , T & Vattulainen , I 2018 , ' How to minimize dye-induced perturbations while studying biomembrane structure and dynamics : PEG linkers as a rational alternative ' , Biochimica et Biophysica Acta. Biomembranes , vol. 1860 , no. 11 , pp. 2436-2445 . https://doi.org/10.1016/j.bbamem.2018.07.003 en
dc.identifier.issn 0005-2736
dc.identifier.other PURE: 117375308
dc.identifier.other PURE UUID: 92066271-5afd-4bfb-babb-b15eaee1cab6
dc.identifier.other RIS: urn:EB36CAA50B09BEB97AE363000484659B
dc.identifier.other Scopus: 85050121034
dc.identifier.other WOS: 000447557500028
dc.identifier.other ORCID: /0000-0003-3377-5753/work/50881955
dc.identifier.other ORCID: /0000-0001-7568-0029/work/50022839
dc.identifier.other ORCID: /0000-0001-7408-3214/work/50022020
dc.identifier.other ORCID: /0000-0003-4858-364X/work/50022934
dc.identifier.uri http://hdl.handle.net/10138/254905
dc.description.abstract Organic dye-tagged lipid analogs are essential for many fluorescence-based investigations of complex membrane structures, especially when using advanced microscopy approaches. However, lipid analogs may interfere with membrane structure and dynamics, and it is not obvious that the properties of lipid analogs would match those of non-labeled host lipids. In this work, we bridged atomistic simulations with super-resolution imaging experiments and biomimetic membranes to assess the performance of commonly used sphingomyelin-based lipid analogs. The objective was to compare, on equal footing, the relative strengths and weaknesses of acyl chain labeling, headgroup labeling, and labeling based on poly-ethyl-glycol (PEG) linkers in determining biomembrane properties. We observed that the most appropriate strategy to minimize dye-induced membrane perturbations and to allow consideration of Brownian-like diffusion in liquid-ordered membrane environments is to decouple the dye from a membrane by a PEG linker attached to a lipid headgroup. Yet, while the use of PEG linkers may sound a rational and even an obvious approach to explore membrane dynamics, the results also suggest that the dyes exploiting PEG linkers interfere with molecular interactions and their dynamics. Overall, the results highlight the great care needed when using fluorescent lipid analogs, in particular accurate controls. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Biochimica et Biophysica Acta. Biomembranes
dc.rights en
dc.subject Fluorescent probe en
dc.subject PEG linker en
dc.subject Lipid membrane en
dc.subject Atomistic simulation en
dc.subject Molecular dynamics simulation en
dc.subject Super-resolution microscopy en
dc.subject 114 Physical sciences en
dc.title How to minimize dye-induced perturbations while studying biomembrane structure and dynamics : PEG linkers as a rational alternative en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1016/j.bbamem.2018.07.003
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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