Biophysical Characterization of Supported Lipid Bilayers Using Parallel Dual-Wavelength Surface Plasmon Resonance and Quartz Crystal Microbalance Measurements

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Parkkila , P , Elderdfi , M , Bunker , A & Viitala , T 2018 , ' Biophysical Characterization of Supported Lipid Bilayers Using Parallel Dual-Wavelength Surface Plasmon Resonance and Quartz Crystal Microbalance Measurements ' , Langmuir , vol. 34 , no. 27 , pp. 8081-8091 . https://doi.org/10.1021/acs.langmuir.8b01259

Title: Biophysical Characterization of Supported Lipid Bilayers Using Parallel Dual-Wavelength Surface Plasmon Resonance and Quartz Crystal Microbalance Measurements
Author: Parkkila, Petteri; Elderdfi, Mohamed; Bunker, Alex; Viitala, Tapani
Contributor: University of Helsinki, Pharmaceutical biophysics group
University of Helsinki, Drug Research Program
University of Helsinki, Faculty of Pharmacy
Date: 2018-07-10
Language: eng
Number of pages: 11
Belongs to series: Langmuir
ISSN: 0743-7463
URI: http://hdl.handle.net/10138/254919
Abstract: Supported lipid bilayers (SLBs) have been used extensively as an effective model of biological membranes, in the context of in vitro biophysics research, and the membranes of liposomes, in the context of the development of nanoscale drug delivery devices. Despite numerous surface-sensitive techniques having been applied to their study, the comprehensive optical characterization of SLBs using surface plasmon resonance (SPR) has not been conducted. In this study, Fresnel multilayer analysis is utilized to effectively calculate layer parameters (thickness and refractive indices) with the aid of dual-wavelength and dispersion coefficient analysis, in which the linear change in the refractive index as a function of wavelength is assumed. Using complementary information from impedance-based quartz crystal microbalance experiments, biophysical properties, for example, area-per-lipid-molecule and the quantity of lipid-associated water molecules, are calculated for different lipid types and mixtures, one of which is representative of a raft-forming lipid mixture. It is proposed that the hydration layer beneath the bilayer is, in fact, an integral part of the measured optical signal. Also, the traditional Jung model analysis and the ratio of SPR responses are investigated in terms of assessing the structure of the lipid layer that is formed.
Subject: 116 Chemical sciences
317 Pharmacy
BIOMOLECULAR ADSORPTION
PHOSPHOLIPID-MEMBRANES
OPTICAL ANISOTROPY
MODEL MEMBRANES
SPECTROSCOPY
VESICLES
ELLIPSOMETRY
DISSIPATION
TEMPERATURE
SCATTERING
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