Cell Membrane-Based Nanoreactor To Mimic the Bio-Compartmentalization Strategy of a Cell

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Balasubramanian , V , Poillucci , A , Correia , A , Zhang , H , Celia , C & Santos , H A 2018 , ' Cell Membrane-Based Nanoreactor To Mimic the Bio-Compartmentalization Strategy of a Cell ' , Acs Biomaterials Science & Engineering , vol. 4 , no. 4 , pp. 1471-1478 . https://doi.org/10.1021/acsbiomaterials.7b00944

Title: Cell Membrane-Based Nanoreactor To Mimic the Bio-Compartmentalization Strategy of a Cell
Author: Balasubramanian, Vimalkumar; Poillucci, Andrea; Correia, Alexandra; Zhang, Hongbo; Celia, Christian; Santos, Helder A.
Contributor organization: Faculty of Pharmacy
Division of Pharmaceutical Chemistry and Technology
Drug Research Program
Preclinical Drug Formulation and Analysis group
Nanomedicines and Biomedical Engineering
Date: 2018-04
Language: eng
Number of pages: 8
Belongs to series: Acs Biomaterials Science & Engineering
ISSN: 2373-9878
DOI: https://doi.org/10.1021/acsbiomaterials.7b00944
URI: http://hdl.handle.net/10138/255648
Abstract: Organelles of eukaryotic cells are structures made up of membranes, which carry out a majority of functions necessary for the surviving of the cell itself. Organelles also differentiate the prokaryotic and eukaryotic cells, and are arranged to form different compartments guaranteeing the activities for which eukaryotic cells are programmed. Cell membranes, containing organelles, are isolated from cancer cells and erythrocytes and used to form biocompatible and long circulating ghost nanoparticles delivering payloads or catalyzing enzymatic reactions as nanoreactors. In this attempt, red blood cell membranes were isolated from erythrocytes, and engineered to form nanoerythrosomes (NERs) of 150 nm. The horseradish peroxidase, used as an enzyme model, was loaded inside the aqueous compartment of NERs, and its catalytic reaction with Resorufm was monitored. The resulting nanoreactor protected the enzyme from proteolytic degradation, and potentiated the enzymatic reaction in situ as demonstrated by maximal velocity (V-max) and Michaelis constant (K-m), thus suggesting the high catalytic activity of nanoreactors compared to the pure enzymes.
Subject: 317 Pharmacy
1182 Biochemistry, cell and molecular biology
nanoparticle reactors
nanoerythrosomes
colloidal nanoparticles
enzyme reaction
self-assembled membrane vesicles
RED-BLOOD-CELLS
DRUG-DELIVERY
POLYMERIC NANOPARTICLES
HORSERADISH-PEROXIDASE
ENZYMATIC-REACTIONS
PARTICLE-SIZE
LIPOSOMES
VESICLES
BIODISTRIBUTION
CATALYSIS
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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