Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants

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Korpela , K , Salonen , A , Vepsäläinen , O , Suomalainen , M , Kolmeder , C , Varjosalo , M , Miettinen , S , Kukkonen , K , Savilahti , E , Kuitunen , M & de Vos , W M 2018 , ' Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants ' , Microbiome , vol. 6 , 182 . https://doi.org/10.1186/s40168-018-0567-4

Title: Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants
Author: Korpela, Katri; Salonen, Anne; Vepsäläinen, Outi; Suomalainen, Marjo; Kolmeder, Carolin; Varjosalo, Markku; Miettinen, Sini; Kukkonen, Kaarina; Savilahti, Erkki; Kuitunen, Mikael; de Vos, Willem M.
Contributor: University of Helsinki, Research Programs Unit
University of Helsinki, Immunobiology Research Program
University of Helsinki, Veterinary Biosciences
University of Helsinki, Max Planck Institute for Developmental Biology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Department of Dermatology, Allergology and Venereology
University of Helsinki, Children's Hospital
University of Helsinki, Clinicum
University of Helsinki, Medicum
Date: 2018-10-16
Language: eng
Number of pages: 11
Belongs to series: Microbiome
ISSN: 2049-2618
URI: http://hdl.handle.net/10138/256399
Abstract: BackgroundInfants born by caesarean section or receiving antibiotics are at increased risk of developing metabolic, inflammatory and immunological diseases, potentially due to disruption of normal gut microbiota at a critical developmental time window. We investigated whether probiotic supplementation could ameliorate the effects of antibiotic use or caesarean birth on infant microbiota in a double blind, placebo-controlled randomized clinical trial. Mothers were given a multispecies probiotic, consisting of Bifidobacterium breve Bb99 (Bp99 2x10(8) cfu) Propionibacterium freundenreichii subsp. shermanii JS (2x10(9)cfu), Lactobacillus rhamnosus Lc705 (5x10(9) cfu) and Lactobacillus rhamnosus GG (5x10(9) cfu) (N=168 breastfed and 31 formula-fed), or placebo supplement (N=201 breastfed and 22 formula-fed) during pregnancy, and the infants were given the same supplement. Faecal samples of the infants were collected at 3months and analyzed using taxonomic, metagenomic and metaproteomic approaches.ResultsThe probiotic supplement had a strong overall impact on the microbiota composition, but the effect depended on the infant's diet. Only breastfed infants showed the expected increase in bifidobacteria and reduction in Proteobacteria and Clostridia. In the placebo group, both birth mode and antibiotic use were significantly associated with altered microbiota composition and function, particularly reduced Bifidobacterium abundance. In the probiotic group, the effects of antibiotics and birth mode were either completely eliminated or reduced.ConclusionsThe results indicate that it is possible to correct undesired changes in microbiota composition and function caused by antibiotic treatments or caesarean birth by supplementing infants with a probiotic mixture together with at least partial breastfeeding.Trial registrationclinicaltrials.gov NCT00298337. Registered March 2, 2006.
Subject: Early-life microbiota
Bifidobacteria
Lactobacilli
Metagenomics
Metaproteomics
INTESTINAL MICROBIOTA
HUMAN-MILK
CONTROLLED-TRIAL
GUT MICROBIOME
IMMUNE-SYSTEM
FOLATE STATUS
1ST YEAR
LIFE
DELIVERY
FORMULA
1183 Plant biology, microbiology, virology
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