Delivery of Agents Into Articular Cartilage With Electric Spark-Induced Sound Waves

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Pérez , A G , Nieminen , H J , Finnilä , M , Salmi , A , Pritzker , K P H , Lampsijärvi , E , Paulin , T , Airaksinen , A J , Saarakkala , S & Haeggström , E 2018 , ' Delivery of Agents Into Articular Cartilage With Electric Spark-Induced Sound Waves ' , Frontiers in Physics , vol. 6 , 116 . https://doi.org/10.3389/fphy.2018.00116

Title: Delivery of Agents Into Articular Cartilage With Electric Spark-Induced Sound Waves
Author: Pérez, Alejandro Garcia; Nieminen, Heikki J.; Finnilä, Mikko; Salmi, Ari; Pritzker, Kenneth P. H.; Lampsijärvi, Eetu; Paulin, Tor; Airaksinen, Anu J.; Saarakkala, Simo; Haeggström, Edward
Contributor organization: Department of Physics
Department of Chemistry
Tracers in Molecular Imaging (TRIM)
Helsinki In Vivo Animal Imaging Platform (HAIP)
Date: 2018-10-16
Language: eng
Number of pages: 7
Belongs to series: Frontiers in Physics
ISSN: 2296-424X
DOI: https://doi.org/10.3389/fphy.2018.00116
URI: http://hdl.handle.net/10138/258271
Abstract: Localized delivery of drugs into articular cartilage (AC) may facilitate the development of novel therapies to treat osteoarthritis (OA). We investigated the potential of spark-gap-generated sound to deliver a drug surrogate, i.e., methylene blue (MB), into AC. In vitro experiments exposed bovine AC samples to either simultaneous sonication and immersion in MB (Treatment 1; n = 10), immersion in MB after sonication (Control 1; n = 10), solely immersion in MB (Control 2; n = 10), or neither sonication nor immersion in MB (Control 3; n = 10). The sonication protocol consisted of 1,000 spark-gap -generated pulses. Delivery of MB into AC was estimated from optical absorbance in transmission light microscopy. Optical absorbance was significantly greater in the treatment group up to 900 mu m depth from AC surface as compared to all controls. Field emission scanning electron microscopy (FESEM), histological analysis, and digital densitometry (DD) of sonicated (n = 6) and non-sonicated (n = 6) samples showed no evidence of sonication-induced changes in proteoglycan content or collagen structure. Consequently, spark-gap -generated sound may offer a solution for localized drug delivery into AC in a non-destructive fashion. Further research on this method may contribute to OA drug therapies.
Subject: osteoarthritis therapies
electric sparks
cartilage
sound
ultrasound
drug delivery
INTRAARTICULAR DRUG-DELIVERY
CELL PORATION
OSTEOARTHRITIS
DISEASE
MODEL
EFFICACY
SYSTEMS
FIELD
DISABILITY
ULTRASOUND
114 Physical sciences
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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