Delivery of Agents Into Articular Cartilage With Electric Spark-Induced Sound Waves

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Pérez , A G , Nieminen , H J , Finnilä , M , Salmi , A , Pritzker , K P H , Lampsijärvi , E , Paulin , T , Airaksinen , A J , Saarakkala , S & Haeggström , E 2018 , ' Delivery of Agents Into Articular Cartilage With Electric Spark-Induced Sound Waves ' , Frontiers in Physics , vol. 6 , 116 . https://doi.org/10.3389/fphy.2018.00116

Title: Delivery of Agents Into Articular Cartilage With Electric Spark-Induced Sound Waves
Author: Pérez, Alejandro Garcia; Nieminen, Heikki J.; Finnilä, Mikko; Salmi, Ari; Pritzker, Kenneth P. H.; Lampsijärvi, Eetu; Paulin, Tor; Airaksinen, Anu J.; Saarakkala, Simo; Haeggström, Edward
Contributor: University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Chemistry
University of Helsinki, Department of Physics
Date: 2018-10-16
Language: eng
Number of pages: 7
Belongs to series: Frontiers in Physics
ISSN: 2296-424X
URI: http://hdl.handle.net/10138/258271
Abstract: Localized delivery of drugs into articular cartilage (AC) may facilitate the development of novel therapies to treat osteoarthritis (OA). We investigated the potential of spark-gap-generated sound to deliver a drug surrogate, i.e., methylene blue (MB), into AC. In vitro experiments exposed bovine AC samples to either simultaneous sonication and immersion in MB (Treatment 1; n = 10), immersion in MB after sonication (Control 1; n = 10), solely immersion in MB (Control 2; n = 10), or neither sonication nor immersion in MB (Control 3; n = 10). The sonication protocol consisted of 1,000 spark-gap -generated pulses. Delivery of MB into AC was estimated from optical absorbance in transmission light microscopy. Optical absorbance was significantly greater in the treatment group up to 900 mu m depth from AC surface as compared to all controls. Field emission scanning electron microscopy (FESEM), histological analysis, and digital densitometry (DD) of sonicated (n = 6) and non-sonicated (n = 6) samples showed no evidence of sonication-induced changes in proteoglycan content or collagen structure. Consequently, spark-gap -generated sound may offer a solution for localized drug delivery into AC in a non-destructive fashion. Further research on this method may contribute to OA drug therapies.
Subject: osteoarthritis therapies
electric sparks
cartilage
sound
ultrasound
drug delivery
INTRAARTICULAR DRUG-DELIVERY
CELL PORATION
OSTEOARTHRITIS
DISEASE
MODEL
EFFICACY
SYSTEMS
FIELD
DISABILITY
ULTRASOUND
114 Physical sciences
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