In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity

Show simple item record

dc.contributor.author Yuryev, Mikhail
dc.contributor.author Andriichuk, Liliia
dc.contributor.author Leiwe, Marcus
dc.contributor.author Jokinen, Ville
dc.contributor.author Carabalona, Aurelie
dc.contributor.author Rivera, Claudio
dc.date.accessioned 2018-11-12T13:31:02Z
dc.date.available 2018-11-12T13:31:02Z
dc.date.issued 2018-10-30
dc.identifier.citation Yuryev , M , Andriichuk , L , Leiwe , M , Jokinen , V , Carabalona , A & Rivera , C 2018 , ' In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity ' , Scientific Reports , vol. 8 , 16059 . https://doi.org/10.1038/s41598-018-34410-x
dc.identifier.other PURE: 118218838
dc.identifier.other PURE UUID: 5f92d64d-8733-47d5-9873-68c994292eb6
dc.identifier.other WOS: 000448745700001
dc.identifier.other Scopus: 85055618563
dc.identifier.other ORCID: /0000-0003-0468-7498/work/50565980
dc.identifier.uri http://hdl.handle.net/10138/260569
dc.description.abstract Prior to sensory experience spontaneous activity appears to play a fundamental role in the correct formation of prominent functional features of different cortical regions. The use of anaesthesia during pregnancy such as ketamine is largely considered to negatively affect neuronal development by interfering with synaptic transmission. Interestingly, the characteristics of spontaneous activity as well as the acute functional effects of maternal anaesthesia remain largely untested in the embryonic cortex in vivo. In the present work, we performed in vivo imaging of spontaneous calcium activity and cell motility in the marginal zone of the cortex of E14-15 embryos connected to the mother. We made use of a preparation where the blood circulation from the mother through the umbilical cord is preserved and fluctuations in intracellular calcium in the embryonic frontal cortex are acquired using two-photon imaging. We found that spontaneous transients were either sporadic or correlated in clusters of neuronal ensembles at this age. These events were not sensitive to maternal isoflurane anaesthesia but were strongly inhibited by acute in situ or maternal application of low concentration of the anaesthetic ketamine (a non-competitive antagonist of NMDA receptors). Moreover, simultaneous imaging of cell motility revealed a correlated strong sensitivity to ketamine. These results show that anaesthetic compounds can differ significantly in their impact on spontaneous early cortical activity as well as motility of cells in the marginal zone. The effects found in this study may be relevant in the etiology of heightened vulnerability to cerebral dysfunction associated with the use of ketamine during pregnancy. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject DEVELOPING MOUSE CORTEX
dc.subject DEVELOPING NEOCORTEX
dc.subject NEURONAL MIGRATION
dc.subject NMDA RECEPTORS
dc.subject CORTICAL INTERNEURONS
dc.subject ELECTRICAL-ACTIVITY
dc.subject WAVES
dc.subject CELLS
dc.subject EXPRESSION
dc.subject MODULATE
dc.subject 3112 Neurosciences
dc.subject 3124 Neurology and psychiatry
dc.title In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity en
dc.type Article
dc.contributor.organization Neuroscience Center
dc.contributor.organization University of Helsinki
dc.contributor.organization Helsinki Institute of Life Science HiLIFE
dc.contributor.organization Helsinki In Vivo Animal Imaging Platform (HAIP)
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1038/s41598-018-34410-x
dc.relation.issn 2045-2322
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
s41598_018_34410_x.pdf 7.213Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record