Transportable system enabling multiple irradiation studies under simultaneous hypoxia in vitro

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dc.contributor.author Metsälä, Olli
dc.contributor.author Kreutzer, Joose
dc.contributor.author Högel, Heidi
dc.contributor.author Miikkulainen, Petra
dc.contributor.author Kallio, Pasi
dc.contributor.author Jaakkola, Panu M.
dc.date.accessioned 2018-12-04T07:53:01Z
dc.date.available 2018-12-04T07:53:01Z
dc.date.issued 2018-11-13
dc.identifier.citation Metsälä , O , Kreutzer , J , Högel , H , Miikkulainen , P , Kallio , P & Jaakkola , P M 2018 , ' Transportable system enabling multiple irradiation studies under simultaneous hypoxia in vitro ' , Radiation oncology , vol. 13 , 220 . https://doi.org/10.1186/s13014-018-1169-9
dc.identifier.other PURE: 119724570
dc.identifier.other PURE UUID: 9c64bc0c-f04d-422c-adcb-e0ab6f069fe7
dc.identifier.other WOS: 000450183900002
dc.identifier.other Scopus: 85056505654
dc.identifier.uri http://hdl.handle.net/10138/270210
dc.description.abstract BackgroundCells in solid tumours are variably hypoxic and hence resistant to radiotherapy - the essential role of oxygen in the efficiency of irradiation has been acknowledged for decades. However, the currently available methods for performing hypoxic experiments in vitro have several limitations, such as a limited amount of parallel experiments, incapability of keeping stable growth conditions and dependence on CO2 incubator or a hypoxia workstation. The purpose of this study was to evaluate the usability of a novel portable system (Minihypoxy) in performing in vitro irradiation studies under hypoxia, and present supporting biological data.Materials and methodsThis study was conducted on cancer cell cultures in vitro. The cells were cultured in normoxic (similar to 21% O-2) or in hypoxic (1% O-2) conditions either in conventional hypoxia workstation or in the Minihypoxy system and irradiated at dose rate 1.28Gy/min2.9%. The control samples were sham irradiated. To study the effects of hypoxia and irradiation on cell viability and DNA damage, western blotting, immunostainings and clonogenic assay were used. The oxygen level, pH, evaporation rate and osmolarity of the culturing media on cell cultures in different conditions were followed.ResultsThe oxygen concentration in interest (5, 1 or 0% O-2) was maintained inside the individual culturing chambers of the Minihypoxy system also during the irradiation. The radiosensitivity of the cells cultured in Minihypoxy chambers was declined measured as lower phosphorylation rate of H2A.X and increased clonogenic capacity compared to controls (OER similar to 3).Conclusions The Minihypoxy system allows continuous control of hypoxic environment in multiple wells and is transportable. Furthermore, the system maintains the low oxygen environment inside the individual culturing chambers during the transportation and irradiation in experiments which are typically conducted in separate facilities. en
dc.format.extent 11
dc.language.iso eng
dc.relation.ispartof Radiation oncology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Hypoxia
dc.subject Cancer
dc.subject Radiotherapy
dc.subject Radiation
dc.subject In vitro
dc.subject Minihypoxy
dc.subject CANCER-CELLS
dc.subject OXYGEN CONCENTRATION
dc.subject TUMOR RADIORESPONSE
dc.subject CULTURE
dc.subject RADIOSENSITIVITY
dc.subject REOXYGENATION
dc.subject CYCLE
dc.subject ENHANCEMENT
dc.subject HIF-1-ALPHA
dc.subject EXPRESSION
dc.subject 3122 Cancers
dc.subject 3126 Surgery, anesthesiology, intensive care, radiology
dc.title Transportable system enabling multiple irradiation studies under simultaneous hypoxia in vitro en
dc.type Article
dc.contributor.organization Department of Oncology
dc.contributor.organization Clinicum
dc.contributor.organization University of Helsinki
dc.contributor.organization HUS Comprehensive Cancer Center
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1186/s13014-018-1169-9
dc.relation.issn 1748-717X
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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